Liquid-liquid partition of radio-labeled substrates between aqueous/organic, two-phase systems ought to give precise and reliable quantitative data. These could then easily analyzed by using established software to yield the desired thermodynamic data such as extraction, distribution, association and stability constants. Mathematical treatment of extraction equilibrium may be applied to achieve information about the composition of the complexes extracted. Furthermore, important parameter such as lipophilicity for the prediction of absorption and distribution of radiotracers in bio-systems can be determined. The radiotracer technique allows precise determination, even at very low concentrations of the species in both phases.
H. Stephan, M. Kubeil, Ke. Gloe, Ka. Gloe
in Analytical Methods in Supramolecular Chemistry, C. A. Schalley (Ed.), 2nd, completely revised and enlarged edition, Wiley-VCH, Weinheim, 2012, 105-125.
M. Bomkamp, C. Siering, K. Landrock, H. Stephan, R. Fröhlich, S. Waldvogel
Extraction of radiolabelled xanthine derivatives by artificial receptors – deep inside into association behaviour
Chem. Eur. J. 2007, 13, 3724-3732.
K. Yoshizuka, H.-J. Pietzsch, S. Seifert, H. Stephan
Quantitative Structure Property Relationship of logP for Radiopharmaceutical Technetium and Rhenium Complexes by using Molecular Dynamics Calculations
Solv. Extr. Res. Devel. (Japan) 2013, 20, 15-27.
P. Comba, B. Martin, A. Sanyal, H. Stephan
The computation of lipophilicities of 64Cu PET systems based on a novel approach for fluctuating charges
Dalton Trans. 2013, 42, 11066-11073.