Generation of high-avidity, WT1-reactive CD8+ cytotoxic T cell clones with anti-leukemic activity by streptamer technology


Generation of high-avidity, WT1-reactive CD8+ cytotoxic T cell clones with anti-leukemic activity by streptamer technology

Tunger, A.; Wehner, R.; von Bonin, M.; Kühn, D.; Heidenreich, F.; Matko, S.; Nauerth, M.; Rücker-Braun, E.; Dietz, S.; Link, C. S.; Eugster, A.; Odendahl, M.; Busch, D. H.; Tonn, T.; Bonifacio, E.; Germeroth, L.; Schetelig, J.; Bachmann, M. P.; Bornhäuser, M.; Schmitz, M.

Summary: In summary, we demonstrated that the expansion of WT1 peptide-specific CD8+ T cells by peptide-loaded MoDCs followed by streptamer-based selection represents an attractive strategy to significantly enrich such T cells prior to cloning. By using this technology, we generated high-avidity, WT1 peptide-reactive CD8+T cell clones with anti-leukemic activity. This strategy may be particular useful for the generation of CD8+T cell clones from healthy donors, in which blood circulating T cells recognizing tumor-associated antigens are rare or not detectable.

Keywords: WT1; CD8+ cytotoxic T cell clones; streptamer technology

Permalink: https://www.hzdr.de/publications/Publ-26305
Publ.-Id: 26305