Helmholtz-Zentrum Dresden-Rossendorf

Biogeochemistry

Dr. Johannes Raff
Phone:+49 351 260 - 2951
Fax:+49 351 260 - 12951
Email: j.raffAthzdr.de
AddressBautzner Landstraße 400
01328 Dresden

Scientific career

  • Since 2017:
    Co-division head biogeochemistry - Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • Since 2016:
    Guest lecturer at Univerity Granada, Spain
  • Since 2015:
    Guest lecturer at Technische Universität Dresden
  • Since 2013:
    Assistant lecturer at University of Applied Sciences, Dresden
  • 2002-2017:
    Research Assistant - Institute of Resource Ecology and since 2011 additionally at Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1997-2002:
    PhD student at University Leipzig/Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1990-1996:
    University Bayreuth, Diploma in Biology

Research work

Investigation of the interaction of radionuclides and industrial metals with bioligands and microorganisms. The aim of the work is to elucidate molecular processes in nature and on the cellular level and to understand their interaction. The results obtained serve on one hand to improve risk assessment and on the other hand for the development of new materials and processes for the detection, removal and recovery of metals.

Projects

  • BioVeStRa, support code 02S9276A, duration 01.06.2016-31.05.2019
  • BioNEWS, support code 03WKCL03F, duration 01.01.2015-30.12.2017
  • S-Sieve, support code 490 ZBG, duration 01.06.2013-29.02.2016
  • Technology transfer project BIOBASE, support code 325/20517289/IMA, duration 01.09.2012-31.03.2013
  • HEF-BioBase, support code HE-2012-04, duration 01.07.2012-30.06.2013
  • Textilien mit S-Layer, support code VF110032, duration 01.06.2012-30.09.2014
  • NanoPharm, support code 03X0094G, duration 01.06.2010-30.09.2013
  • ForMaT: BioNano, support code 03FO3141, duration 28.09.2009-31.03.2010
  • BIOREM, support code 03EGSSN014, duration 01.04.2008-31.03.2009
  • Urantransport, support code 02S8517, duration 01.10.2007-30.09.2010
  • NanoFoto, support code 01SF0717, duration 01.10.2007-31.12.2008

Publications

2017

Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling
Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.
Corresponding author: Pollmann, K.
  • Poster
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Contribution to proceedings
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Open Access LogoSolid State Phenomena 262(2017), 389-393
    DOI-Link: http://dx.doi.org/10.4028/www.scientific.net/SSP.262.389

Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus
Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.

2016

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources
Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.
Corresponding author: Pollmann, K.

Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins
Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.
Corresponding author: Pollmann, K.

Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes
Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.

Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies
Suhr, M.; Raff, J.; Pollmann, K.

2015

Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate
Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces
Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.

Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis
Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.

S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces
Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM
Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.

Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles
Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.

2014

Immobilization of microorganisms for AFM studies in liquids
Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP
Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.

Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods
Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.

S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability
Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.

2013

Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes
Weinert, U.; Pollmann, K.; Raff, J.

Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53
Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.

2012

Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells
Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.

2011

E. coli filament formation induced by heterologous S-layer expression
Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.

2010

Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity
Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.

Self assembling proteins as technology platform for the development of new bio-composite materials
Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
  • Lecture (Conference)
    12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland
  • Materialwissenschaft und Werkstofftechnik (2010)

Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach
Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.
  • Journal of Inorganic Biochemistry 104(2010)7, 718-725

Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane
Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.

2008

Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris
Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.

2007

Magnetic properties of transition-metal nanoclusters on a biological substrate
Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.
  • Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
  • Lecture (Conference)
    International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan

2006

Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy
Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.

Toxic avengers
Raff, J.; Selenska-Pobell, S.
  • Nuclear Engineering International 51(2006)619, 34-36

Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells
Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.
  • Letters in Applied Microbiology 43(2006)1, 39-45

Metal binding by bacteria from uranium mining waste piles and its technological applications
Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.

2005

Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements
Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.

Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12
Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.

2004

Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers
Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.

Bacteria from extreme environments: basis for new technical applications
Raff, J.; Selenska-Pobell, S.
  • BioMeT-Magazin (2004), 18

Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies
Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
  • Lecture (Conference)
    14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark
  • Geochimica et Cosmochimica Acta 68(2004)11, 499-499
  • Contribution to proceedings
    14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark
    Proceedings

Use of Bacteria for Cleaning Radioactive Wastewaters
Raff, J.; Selenska-Pobell, S.
  • Genomics/Proteomics Technology 22(2004)2, 32L-32N

2003

Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers
Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.
  • Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)

Biosorption of Uranium and Copper by Biocers
Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.
  • Chemistry of Materials, 15 (1), 240-244, Jan. 2003

2001

A fast screening method for surface layers on Gram-positive bacteria
Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.
  • Biotechnology letters 23, 1485-1490 (2001)

Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602
Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.
  • Advanced Materials 2001, 13, No. 10, May 17, p. 736-740

EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces
Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.
  • Radiochimica Acta 89 (2001), 625-631

2000

Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile
Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.
  • Radiochimica Acta 88, 71-76 (2000)