Helmholtz-Zentrum Dresden-Rossendorf

Biogeochemistry

Dr. Johannes Raff
Phone:+49 351 260 - 2951
Fax:+49 351 260 - 12951
Email: j.raffAthzdr.de
Address for visitors:Bautzner Landstraße 400
01328 Dresden
Postal address:POB 51 01 19
01314 Dresden
Germany

Scientific career

  • Since 2017:
    Co-division head biogeochemistry - Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • Since 2016:
    Guest lecturer at Univerity Granada, Spain
  • Since 2015:
    Guest lecturer at Technische Universität Dresden
  • Since 2013:
    Assistant lecturer at University of Applied Sciences, Dresden
  • 2002-2017:
    Research Assistant - Institute of Resource Ecology and since 2011 additionally at Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1997-2002:
    PhD student at University Leipzig/Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1990-1996:
    University Bayreuth, Diploma in Biology

Research work

Investigation of the interaction of radionuclides and industrial metals with bioligands and microorganisms. The aim of the work is to elucidate molecular processes in nature and on the cellular level and to understand their interaction. The results obtained serve on one hand to improve risk assessment and on the other hand for the development of new materials and processes for the detection, removal and recovery of metals.

Projects (Head and/or coordination)

  • BioVeStRa, support code 02S9276A, duration 01.06.2016-31.05.2019
  • BioNEWS, support code 03WKCL03F, duration 01.01.2015-30.12.2017
  • S-Sieve, support code 490 ZBG, duration 01.06.2013-29.02.2016
  • Technology transfer project BIOBASE, support code 325/20517289/IMA, duration 01.09.2012-31.03.2013
  • HEF-BioBase, support code HE-2012-04, duration 01.07.2012-30.06.2013
  • Textilien mit S-Layer, support code VF110032, duration 01.06.2012-30.09.2014
  • NanoPharm, support code 03X0094G, duration 01.06.2010-30.09.2013
  • ForMaT: BioNano, support code 03FO3141, duration 28.09.2009-31.03.2010
  • BIOREM, support code 03EGSSN014, duration 01.04.2008-31.03.2009
  • Urantransport, support code 02S8517, duration 01.10.2007-30.09.2010
  • NanoFoto, support code 01SF0717, duration 01.10.2007-31.12.2008

Publications

2017

Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.
Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus

2016

Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.
Corresponding author: Pollmann, K.
Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.
Corresponding author: Pollmann, K.
Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins

Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.
Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes

Suhr, M.; Raff, J.; Pollmann, K.
Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies

2015

Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.
Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate

Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.
Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.
Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis

Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.
S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces

Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.
Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM

Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.
Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles

2014

Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.
Immobilization of microorganisms for AFM studies in liquids

Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.
Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.
Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.
Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.
S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability

2013

Weinert, U.; Pollmann, K.; Raff, J.
Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes

Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.
Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

2012

Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells

2011

Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.
E. coli filament formation induced by heterologous S-layer expression

2010

Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.
Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
Self assembling proteins as technology platform for the development of new bio-composite materials
  • Lecture (Conference)
    12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland
  • Materialwissenschaft und Werkstofftechnik (2010)

Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.
Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach
  • Journal of Inorganic Biochemistry 104(2010)7, 718-725

Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.
Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane

2008

Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.
Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris

2007

Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.
Magnetic properties of transition-metal nanoclusters on a biological substrate
  • Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
  • Lecture (Conference)
    International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan

2006

Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.
Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy

Raff, J.; Selenska-Pobell, S.
Toxic avengers
  • Nuclear Engineering International 51(2006)619, 34-36

Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.
Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells
  • Letters in Applied Microbiology 43(2006)1, 39-45

Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.
Metal binding by bacteria from uranium mining waste piles and its technological applications

2005

Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.
Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12

2004

Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.
Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers

Raff, J.; Selenska-Pobell, S.
Bacteria from extreme environments: basis for new technical applications
  • BioMeT-Magazin (2004), 18

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies
  • Lecture (Conference)
    14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark
  • Geochimica et Cosmochimica Acta 68(2004)11, 499-499
  • Contribution to proceedings
    14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark
    Proceedings

Raff, J.; Selenska-Pobell, S.
Use of Bacteria for Cleaning Radioactive Wastewaters
  • Genomics/Proteomics Technology 22(2004)2, 32L-32N

2003

Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.
Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers
  • Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)

Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.
Biosorption of Uranium and Copper by Biocers
  • Chemistry of Materials, 15 (1), 240-244, Jan. 2003

2001

Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.
A fast screening method for surface layers on Gram-positive bacteria
  • Biotechnology letters 23, 1485-1490 (2001)

Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.
Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602
  • Advanced Materials 2001, 13, No. 10, May 17, p. 736-740

Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.
EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces
  • Radiochimica Acta 89 (2001), 625-631

2000

Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.
Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile
  • Radiochimica Acta 88, 71-76 (2000)