Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf

1 Publication
The response of a halophilic microbial isolate from rock salt to repository relevant conditions
Bachran, M.; Hilpmann, S.; Kluge, S.; Cherkouk, A.;
Next to crystalline rock and clay, rock salt formations are considered as potential host rock systems for the long-term storage of highly radioactive waste in a deep geological repository in Germany. To date, little is known about the habitat rock salt and the way of life of the microorganisms occurring there. Due to the high salinity and lack of nutrients, only adapted microorganisms such as extremely halophilic archaea can survive under these extreme conditions.[1] It is of interest to know what kind of extreme halophilic archaea are living there, how active they are under repository relevant conditions, and how these microorganisms can influence the safe storage of the waste. In this study an Halobacterium isolate was retrieved from a German rock salt sample. This Halobacterium sp. GP5 1-1 was investigated in detail with regard to its interaction with uranium, one of the major radionuclides in highly radioactive wastes. In comparison to Halobacterium noricense DSM 15987T the studied species showed only the involvement of carboxylate groups in the interaction with uranium by in situ attenuated total reflection fourier-transform infrared spectroscopy.[2] In addition, information about the activity and possible metabolism under repository relevant conditions of Halobacterium sp. GP5 1-1 were gain via proteomic analysis. Finally, microbial diversity studies based on the isolation of high-molecular weight genomic DNA from German rock salt samples and the subsequent high-throughput sequencing of the V4 region of the 16S rRNA gene were performed.
  • Poster
    12th Edition of the International Congress on Extremophiles, 16.-20.09.2018, Ischia, Italien

Publ.-Id: 27480 - Permalink