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Not just a background: pH buffers do interact with lanthanide ions – a Europium(III) case study

Mandal, P.; Kretzschmar, J.; Drobot, B.

The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry viz. HEPES, PIPES, MES, MOPS, and TRIS have been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good’s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good’s buffers. 

Keywords: Europium; Buffer; TRLFS; NMR; Stability Constant

  • Software in the HZDR data repository RODARE
    Publication date: 2021-09-09
    DOI: 10.14278/rodare.1147
    License: CC-BY-4.0

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Permalink: https://www.hzdr.de/publications/Publ-33100
Publ.-Id: 33100