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Stability of 188Re Complexes Prepared with Highly Concentrated [188Re]Perrhenate Eluates from 188W/188Re GeneratorsSeifert, S.; Schiller, E.; Jentschel, C.; Bergmann, R.; Pietzsch, H.-J.; Wunderlich, G.; Kotzerke, J.; Steinbach, J.
The preparation and stability of a novel 188Re-S4-complex (S4 = bridged DMSA ligand) was studied at therapeutic relevant radioactive concentrations. The results were compared with an 188Re-MAG3 and an 188Re-DMSA preparation which were obtained from 188Re-EDTA by a combined re-oxidation/ligand exchange reaction. The highly concentrated [188Re]perrhenate solution (12-15 GBq/ml) used for preparations was received by successive elution of several 188W/188Re generators connected in series. The preparations were performed under nitrogen, stabilized by adding 15 mg of ascorbic acid and analyzed after 1, 2, and 24 hours by TLC and HPLC. Only about 8 % of perrhenate were detected after 24 h in the 188Re-S4-complex samples. The 188Re-DMSA preparations were only marginally re-oxidized during this time, whereas the stabilized 188Re-MAG3 samples remained nearly stable. Additionally, in vitro and in vivo stability studies at radioactive concentrations of 30-50 MBq/ml were performed with all the complexes. Therefore, the preparations were purified by HPLC and incubated in phosphate buffer as well as rat and human plasma. The in-vivo-stability was determined in rats. Samples of plasma, urine, liver, and intestine were taken off 1 h after application of the 188Re complex solutions. Results from these studies demonstrated that only the 188Re-S4-complex was completely stable in vitro and in all investigated in vivo samples.
7th International Symposium on Technetium in Chemistry and Nuclear Medicine, 06.-09.09.2006, Bressanone, Italy
Contribution to external collection
U. Mazzi: Technetium, Rhenium and Other Metals in Chemistry and Nuclear Medicine, Padova: SGEditoriali, 2006, 88-89884-04-5, 605-606