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Proton single voxel spectroscopy of different adipose tissue deposits in mice

Strobel, K.; van den Hoff, J.; Pietzsch, J.
Accumulation of excess abdominal visceral fat has been shown to play crucial roles in the development of cardiovascular disease as well as the development of obesity-related disorders like Diabetes mellitus, hyperlipidemia, and hypertension.
In the last years, rodent models have gained an important role in the investigation of adipose tissue (AT) biology and disorder. In this line, non-invasive differentiation and characterization of various AT deposits in the living animal is a current challenge. Magnetic resonance spectroscopy (MRS) at high magnetic field strength can help us to learn more about lipids in vivo. MRS is applied for brown adipose tissue (BAT), as well as intra-abdominal white adipose tissue (WAT) deposits in mice.
All experiments were carried out in NMRI mice and nude mice. Morphological differentiation between various BAT and WAT deposits was obtained by 1H-MRI at 7 Tesla using a Biospec 70/30 (Bruker, Germany). 1H-MRS has been performed to quantify in vivo the different lipid patterns in BAT and WAT deposits using a volume selective PRESS sequence on 1 to 8 mm3 voxels.
In both nude mice and NMRI mice the various BAT and WAT deposits were clearly distinguished from the non-AT tissue with excellent contrast by T1-weighted MSME MRI sequences. The high resolution spectra obtained at 7 T allow identification of at least 9 different proton resonances specific for lipids, and, thus, for calculation of mono- to polyunsaturated fatty acid ratio in vivo. In this study, nude mice showed a 3-fold higher degree of polyunsaturation of triglyceride fatty acid acyl chains in BAT when compared to NMRI mice. No significant differences were observed in WAT deposits.
High-resolution MRS is a potentially useful tool for studying the biology of different BAT and WAT deposits non-invasively in small experimental animals in vivo.
  • Lecture (others)
    User Meeting 2006 der Fa. Bruker Biospin MRI GmbH, 09.-12.10.2006, Ettlingen, Germany

Publ.-Id: 8978