Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf
Heavy Metal Binding Peptides – Design and Construction of new Biosorbents
Braun, R.; Schönberger, N.; Bachmann, S.; Matys, S.; Lederer, F.; Pollmann, K.
Heavy metal contaminations in both industrial and environmental waters are widely occurring. However, removal is both challenging and cost-intensive. In this study, we identified metal-binding peptide sequences using phage surface display (PSD). Fusion proteins with PSD-derived sequences were construced for further recombinant production, future scale-up and as alternative to chemical synthesis. The construction of the fusion proteins included usage of inteins and affinity tags for simplified expression and purification. Quartz crystal microbalance with dissipation monitoring (QCM-D) was used for further characterization of the peptide-metal interaction. The system developed in this study provides metal-binding peptides with high specificity and sensitivity. Being biodegradable, the constructed peptides can be used in multiple applications. The identified motifs can furthermore provide a deeper understanding of peptide-metal interaction, leading to the discovery of novel metal-interacting biomolecules and better prediction of involved amino acids.
8th Peptide Engineering Meeting, 08.-10.11.2018, Berlin, Deutschland