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Effect of increase of radiation dose on local control relates to pre-treatment FDG uptake in FaDu tumours in nude mice

Sch├╝tze, C.; Bergmann, R.; Yaromina, A.; Hessel, F.; Kotzerke, J.; Steinbach, J.; Baumann, M.; Beuthien-Baumann, B.

Objectives:

To investigate whether heterogeneity in [18F]2-fluoro-2-deoxy-D-glucose (FDG) uptake in a single tumour line, i.e. in tumours with identical genetic background, relates to radiation response.

Materials and methods:

Sixty-two human FaDu head and neck squamous cell carcinomas in nude mice with a diameter of 7 mm entered the study. FDG-PET scanning was performed without anaesthesia on an animal PET scanner immediately prior to irradiation in order to determine maximum standardized uptake values (SUVmax). Single dose irradiations of 25 or 35 Gy were applied under normal blood flow conditions using 200 kV X-rays (0.5 mm Cu, ~ 1.2 Gy min-1). The mice
were observed for 120 days after irradiation, experimental endpoint was local tumour control evaluated using the
Kaplan-Meier method.

Results:

Analyzing all 62 animals, tumour control probability after irradiation with 25 Gy was significantly lower than after irradiation with 35 Gy (29% vs. 57%, log rank p = 0.016). Pre-treatment SUVmax values ranged from 0.72 to 3.47, the median SUVmax value was 1.59. In tumours with FDG uptake less than the median SUVmax, local control was 37% after 25 Gy vs. 47% after 35 Gy (p = 0.37). In contrast, substantial differences in local tumour control were found in tumours with FDG uptake above the median SUVmax (24% vs. 71%, p = 0.006). Multivariate Cox analysis revealed a significant decrease of hazard of recurrence with increasing dose and SUVmax.

Conclusions:

An increase of radiation dose had a greater effect on local control in FaDu tumours with higher FDG uptake than in tumours with lower FDG uptake. This supports the hypothesis that pre-treatment FDG-PET may provide
useful information for heterogeneous radiation dose prescription in subvolumes of tumours of individual patients. As only one tumour model was studied and single doses were applied, confirmatory investigations using further tumour models and fractionated radiotherapy are warranted.

Keywords: (18F]FDG; Positron emission tomography; Irradiation; Squamous cell carcinoma; Human tumour xenografts; Local tumour control

Permalink: https://www.hzdr.de/publications/Publ-9983
Publ.-Id: 9983