Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf

1 Publication

Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass

Schrader, M.; Bobeth, C.; Lederer, F.

The increasing complexity and need of high-tech materials for modern electronics raises the demand for rare earth elements. While recycling rates are still negligible for most elements, geopolitical tensions, circular economy and the aim for a carbon neutral society puts pressure on conventional supply strategies and emphasize the need for new ideas for recycling. Our research group works on the development of phage surface display (PSD) derived peptide based recycling methods for electronic waste. This study focuses on LaPO4:Ce,Tb (LAP), a component of electronic waste from compact energy saving lamps containing rare earth elements enriched fluorescent powders. While free solution phase peptides show little to no interaction with the target material, we re-enabled the binding capability by immobilizing them on various glass supports. We shine a spotlight on the transition from phage bound to free peptides and present the first proof of successful peptide-LAP particle interactions of previously reported PSD derived sequences. Therefore, we introduce a method to investigate qualitatively and quantitatively peptide-particle-interactions. Additionally, a calibration curve allowed the quantification of peptide bound particles. Combined with the quantification of the immobilized peptide on the surface it was possible to calculate a potential dosage of peptides for future recycling processes.

Keywords: Phage Surface Display; Peptide; Immobilization; Interaction; Glass Functionalization; Binding; Particle; Fluorescent Lamp Powder; Recycling; Fluorescence scanning

Related publications

Permalink: https://www.hzdr.de/publications/Publ-31119
Publ.-Id: 31119