Contact

Dr. Johannes Raff
Head Biogeochemistry
j.raffAthzdr.de
Phone: +49 351 260 2951
Fax: +49 351 260 12951

Recent publications

2019

Thermodynamic and structural studies on the Ln(III)/An(III) malate complexation
Taube, F.; Drobot, B.; Roßberg, A.; Foerstendorf, H.ORC; Acker, M.; Patzschke, M.; Trumm, M.; Taut, S.; Stumpf, T.
Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon
Bader, M.; Moll, H.; Steudtner, R.; Lösch, H.; Drobot, B.; Stumpf, T.; Cherkouk, A.;
Microbial diversity in an arid, naturally saline environment
Bachran, M.; Kluge, S.; Lopez-Fernandez, M.; Cherkouk, A.;
Synthesis, 18F-labelling and radiopharmacological characterisation of the C-terminal 30mer of Clostridium perfringens enterotoxin as a potential claudin-targeting peptide
Löser, R.; Bader, M.; Kuchar, M.; Wodtke, R.; Lenk, J.; Wodtke, J.; Kuhne, K.; Bergmann, R.; Haase-Kohn, C.; Urbanová, M.; Steinbach, J.; Pietzsch, J.;
Reversible pH-dependent curium(III) biosorption by the bentonite yeast isolate Rhodotorula mucilaginosa BII-R8
Lopez-Fernandez, M.; Moll, H.; Merroun, M. L.;

2018

Depth and Dissolved Organic Carbon Shape Microbial Communities in Surface Influenced but Not Ancient Saline Terrestrial Aquifers
Lopez-Fernandez, M.; Åström, M.; Bertilsson, S.; Dopson, M.;
Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells
Ni, G.; Canizales, S.; Broman, E.; Simone, D.; Palwai, V. R.; Lundin, D.; Lopez-Fernandez, M.; Sleutels, T.; Dopson, M.;
Metatranscriptomes Reveal That All Three Domains of Life Are Active but Are Dominated by Bacteria in the Fennoscandian Crystalline Granitic Continental Deep Biosphere
Lopez-Fernandez, M.; Simone, D.; Wu, X.; Soler, L.; Nilsson, E.; Holmfeldt, K.; Lantz, H.; Bertilsson, S.; Dopson, M.;
Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling
Lopez-Fernandez, M.; Broman, E.; Turner, S.; Wu, X.; Bertilsson, S.; Dopson, M.;
Impact of Haloarchaea on speciation of uranium – a multi-spectroscopic approach
Bader, M.; Rossberg, A.; Steudtner, R.; Drobot, B.; Großmann, K.; Schmidt, M.; Musat, N.; Stumpf, T.; Ikeda-Ohno, A.; Cherkouk, A.;
Ultrafast transient absorption spectroscopy of UO22+ and [UO2Cl]+
Haubitz, T.; Tsushima, S.; Steudtner, R.; Drobot, B.; Geipel, G.; Stumpf, T.; Kumke, M. U.;
Effect of U(VI) aqueous speciation on the binding of uranium by the cell surface of Rhodotorula mucilaginosa, a natural yeast isolate from bentonites
Lopez-Fernandez, M.; Romero-Gonzalez, M.; Günther, A.; Solari, P. L.; Merroun, M. L.;
Metabolism-dependent bioaccumulation of uranium by Rhodosporidium toruloides isolated from the flooding water of a former uranium mine
Gerber, U.; Hübner, R.; Rossberg, A.; Krawczyk-Bärsch, E.; Merroun, M. L.;
Removal and recovery of uranium by waste digested activated sludge in fed-batch stirred tank reactor
Jain, R.; Peräniemi, S.; Jordan, N.; Vogel, M.; Weiss, S.; Foerstendorf, H.; Lakaniemi, A. M.;
Bio-recycling of metals: Recycling of technical products using biological applications
Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.;
Multidisciplinary characterization of U(VI) sequestration by Acidovorax facilis for bioremediation purposes
Krawczyk-Bärsch, E.; Gerber, U.; Müller, K.; Moll, H.; Rossberg, A.; Steudtner, R.; Merroun, M.;
Comparative analysis of uranium bioassociation with halophilic bacteria and archaea
Bader, M.; Müller, K.; Foerstendorf, H.; Schmidt, M.; Simmons, K.; Swanson, J. S.; Reed, D. T.; Stumpf, T.; Cherkouk, A.;
Biotransformation and detoxification of selenite by microbial biogenesis of selenium-sulfur nanoparticles
Vogel, M.; Fischer, S.; Maffert, A.; Hübner, R.; Scheinost, A.; Franzen, C.; Steudtner, R.;

2017

Calorimetrically determined U(VI) toxicity in Brassica napus correlates with oxidoreductase activity and U(VI) speciation
Sachs, S.; Geipel, G.; Bok, F.; Oertel, J.; Fahmy, K.;
Phage display – a new tool for the recovery of valuable metals from primary and secondary resources
Matys, S.; Lederer, F.; Schönberger, N.; Braun, R.; Lehmann, F.; Flemming, K.; Bachmann, S.; Curtis, S.; Macgillivray, R.; Pollmann, K.;
  • Open Access LogoSolid State Phenomena 262(2017), 443-446
    DOI: 10.4028/www.scientific.net/SSP.262.443
  • Lecture (Conference)
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling
Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.;
  • Poster
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Contribution to proceedings
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Open Access LogoSolid State Phenomena 262(2017), 389-393
    DOI: 10.4028/www.scientific.net/SSP.262.389
The occurrences of Ca2UO2(CO3)3 in Fe(II) containing deep groundwater at Forsmark, eastern Sweden.
Tullborg, E.-L.; Suksi, J.; Geipel, G.; Krall, L.; Auqué, L.; Gimeno, M.; Puigdomenech, I.;
Plutonium interaction studies with the Mont Terri Opalinus Clay isolate Sporomusa sp. MT-2.99: changes in the plutonium speciation by solvent extractions.
Moll, H.; Cherkouk, A.; Bok, F.; Bernhard, G.;
Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques
Bader, M.; Müller, K.; Foerstendorf, H.; Drobot, B.; Schmidt, M.; Musat, N.; Swanson, J. S.; Reed, T. D.; Stumpf, T.; Cherkouk, A.;
Leaching of Rare Earth Elements from fluorescent powder using the tea fungus Kombucha
Hopfe, S.; Flemming, K.; Lehmann, F.; Möckel, R.; Kutschke, S.; Pollmann, K.;
Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus
Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.;

2016

Uranium contaminated drinking water linked to leukaemia – revisiting a case study from South Africa taking alternative exposure pathways into account
Winde, F.; Erasmus, E.; Geipel, G.;
Combined use of flow cytometry and microscopy to study the interactions between the gram-negative betaproteobacterium Acidovorax facilis and uranium(VI)
Gerber, U.; Zirnstein, I.; Krawczyk-Bärsch, E.; Lünsdorf, H.; Arnold, T.; Merroun, M. L.;
Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources
Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.;
Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins
Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.;
Study of the Interaction of Eu3+ with Microbiologically Induced Calcium Carbonate Precipitates using TRLFS
Johnstone, E. V.; Hofmann, S.; Cherkouk, A.; Schmidt, M.;
Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes
Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.;
Macroscopic and spectroscopic characterization of uranium(VI) sorption onto orthoclase and muscovite and the influence of competing Ca2+
Richter, C.; Müller, K.; Drobot, B.; Steudtner, R.; Großmann, K.; Stockmann, M.; Brendler, V.;
Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies
Suhr, M.; Raff, J.; Pollmann, K.;
  • Journal of Visualized Experiments 107(2016), e53572
    DOI: 10.3791/53572

2015

Phenol degradation by environmental bacteria entrapped in cryogels
Satchanska, G.; Topalova, Y.; Dimkov, R.; Groudeva, V.; Petrov, P.; Tsvetanov, C.; Selenska-Pobell, S.; Golovinsky, E.;
The interaction of Eu(III) with organoborates – a further approach to understand the complexation in the An/Ln(III)–borate system
Schott, J.; Kretzschmar, J.; Tsushima, S.; Drobot, B.; Acker, M.; Barkleit, A.; Taut, S.; Brendler, V.; Stumpf, T.;
Bacterial diversity in bentonites, engineered barrier for deep geological disposal of radioactive wastes
Lopez-Fernandez, M.; Cherkouk, A.; Vílchez-Vargas, R.; Sandoval, R.; Pieper, D.; Boon, N.; Sánchez-Castro, I.; Merroun, M.;
Interaction of Eu(III) with mammalian cells: Cytotoxicity, uptake and speciation as a function of Eu(III) concentration and nutrient composition
Sachs, S.; Heller, A.; Weiß, S.; Bok, F.; Bernhard, G.;
Speciation of uranium in compartments of living cells
Geipel, G.; Viehweger, K.;
Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces
Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.;
Uranium(VI) Binding Forms in Selected Human Body Fluids: Thermodynamic Calculations versus Spectroscopic Measurements
Osman, A. A. A.; Geipel, G.; Barkleit, A.; Bernhard, G.;
Spectroscopic study on uranyl carboxylate complexes formed at the surface layer of Sulfolobus acidocaldarius
Reitz, T.; Rossberg, A.; Barkleit, A.; Steudtner, R.; Selenska-Pobell, S.; Merroun, M. L.;
Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis
Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.;
S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces
Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.;
Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM
Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.;
A spectroscopic study on U(VI) biomineralization in cultivated Pseudomonas fluorescens biofilms isolated from granitic aquifers
Krawczyk-Bärsch, E.; Lütke, L.; Moll, H.; Bok, F.; Steudtner, R.; Rossberg, A.;
Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles
Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.;

2014

Decrease of U(VI) immobilization capability of the facultative anaerobic strain Paenibacillus sp. JG TB8 under anoxic conditions due to strongly reduced phosphatase activity.
Reitz, T.; Rossberg, A.; Barkleit, A.; Selenska-Pobell, S.; Merroun, M. L.;
Phylogenetic diversity of archaea and the archaeal ammonia monooxygenase gene in uranium mining-impacted locations in Bulgaria
Radeva, G.; Kenarova, A.; Bachvarova, V.; Flemming, K.; Popov, I.; Vassilev, D.; Selenska-Pobell, S.;
EGF receptor-targeting peptide conjugate incorporating a near-IR fluorescent dye and a novel 1,4,7-triazacyclononane-based 64Cu(II) chelator assembled via click chemistry
Viehweger, K.; Barbaro, L.; Pombo García, K.; Joshi, T.; Geipel, G.; Steinbach, J.; Stephan, H.; Spiccia, L.; Graham, B.;
Biosorption and Biomineralization of U(VI) by the Marine Bacterium Idiomarina loihiensis MAH1: Effect of Background Electrolyte and pH
Morcillo, F.; Gonzalez-Munoz, M. T.; Reitz, T.; Romero-Gonzalez, M. E.; Arias, J. M.; Merroun, M. L.;
Immobilization of microorganisms for AFM studies in liquids
Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.;
Formation of a Eu(III) borate solid species from a weak Eu(III) borate complex in aqueous solution
Schott, J.; Kretzschmar, J.; Acker, M.; Eidner, S.; Kumke, M. U.; Drobot, B.; Barkleit, A.; Taut, S.; Brendler, V.; Stumpf, T.;
Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie
Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.;
Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP
Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.;
Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods
Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.;
Uranium(VI) Chemistry in Strong Alkaline Solution: Speciation and Oxygen Exchange Mechanism
Moll, H.; Rossberg, A.; Steudtner, R.; Drobot, B.; Müller, K.; Tsushima, S.;
Uranium contents in plants and mushrooms grown on a uranium contaminated site near Ronneburg in Eastern Thuringia/Germany
Baumann, N.; Arnold, T.; Haferburg, G.;
Interactions of the Mont Terri Opalinus Clay isolate Sporomusa sp. MT-2.99 with curium(III) and europium(III)
Moll, H.; Lütke, L.; Geissler, A.; Selenska-Pobell, S.; Bernhard, G.; Bachvarova, V.;
Speciation of bioaccumulated uranium(VI) by Euglena mutabilis cells obtained by laser fluorescence spectroscopy
Brockmann, S.; Arnold, T.; Bernhard, G.;
S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability
Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.;
How plants cope with heavy metals
Viehweger, K.;

2013

Bacterial Diversity at Abandoned Uranium Mining and Milling Sites in Bulgaria as Revealed by 16S rRNA Genetic Diversity Study
Radeva, G.; Kenarova, A.; Bachvarova, V.; Flemming, K.; Popov, I.; Vassilev, D.; Selenska-Pobell, S.;
Mobile uranium(IV)-bearing colloids in a mining-impacted wetland
Wang, Y.; Frutschi, M.; Suvorova, E.; Phrommavanh, V.; Descostes, M.; Osman, A. A. A.; Geipel, G.; Bernier-Latmani, R.;
Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes
Weinert, U.; Pollmann, K.; Raff, J.;
Uptake of multi-disperse starch by anion exchangers
Pürschel, M.; Sachs, S.; Bernhard, G.; Ender, V.;
  • Open Access LogoJournal of Physical Science and Application 3(2013)3, 147-155
Spectroscopic study of americium(III) complexes with nitrogen containing organic model ligands
Raditzky, B.; Sachs, S.; Schmeide, K.; Barkleit, A.; Geipel, G.; Bernhard, G.;
Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53
Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.;
Investigation of Uranium Binding Forms in Some German Mineral Waters
Osman, A. A. A.; Geipel, G.; Bernhard, G.; Worch, E.;
The U(VI) Speciation Influenced by a Novel Paenibacillus Isolate from Mont Terri Opalinus Clay
Lütke, L.; Moll, H.; Bachvarova, V.; Selenska-Pobell, S.; Bernhard, G.;
Diffusion of U(VI) in Opalinus Clay: Influence of temperature and humic acid
Joseph, C.; van Loon, L. R.; Jakob, A.; Steudtner, R.; Schmeide, K.; Sachs, S.; Bernhard, G.;
Interaction of Uranium(VI) with Bioligands Present in Human Biological Fluids: The Case Study of Urea and Uric Acid
Osman, A. A. A.; Geipel, G.; Bernhard, G.;
Chitin-based Renewable Materials from Marine ponges for Uranium Adsorption
Schleuter, D.; Günther, A.; Paasch, S.; Ehrlich, H.; Kljajic, Z.; Hanke, T.; Bernhard, G.; Brunner, E.;
Sorption of U(VI) onto Opalinus Clay: Effects of pH and humic acid
Joseph, C.; Stockmann, M.; Schmeide, K.; Sachs, S.; Brendler, V.; Bernhard, G.;
Curium(III) speciation studies with cells of a groundwater strain of Pseudomonas fluorescens
Moll, H.; Lütke, L.; Barkleit, A.; Bernhard, G.;

2012

Strong paramagnetism of Gold nanoparticles deposited on a Sulfolobus acidocaldarius S-layer
Bartolome, J.; Bartolome, F.; Garcia, L. M.; Figueroa, A. I.; Repolles, A.; Martinez, M. J.; Luis, F.; Magen, C.; Selenska-Pobell, S.; Pobell, F.; Reitz, T.; Schönemann, T.; Herrmannsdörfer, T.; Merroun, M.; Geissler, A.; Wilhelm, F.; Rogalev, A.;
Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells
Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.;
Insights on the Uranium(VI) Speciation with Pseudomonas fluorescens on a Molecular Level
Lütke, L.; Moll, H.; Bernhard, G.;
Uranyl(VI) Complexation by Sulfonate Ligands: A Relativistic Density Functional and TRLFS Study
Kremleva, A.; Zhang, Y.; Shor, A. M.; Krüger, S.; Joseph, C.; Raditzky, B.; Schmeide, K.; Sachs, S.; Bernhard, G.; Rösch, N.;
Implementation of microbial processes in the performance assessment of spent nuclear fuel repositories
Behrends, T.; Krawczyk-Bärsch, E.; Arnold, T.;
Immobilization of uranium in biofilm microorganisms exposed to groundwater seeps over granitic rock tunnel walls in Olkiluoto, Finland
Krawczyk-Bärsch, E.; Lünsdorf, H.; Arnold, T.; Bok, F.; Steudtner, R.; Pedersen, K.; Lehtinen, A.; Brendler, V.;
Np(V) reduction by humic acid: Contribution of reduced sulfur functionalities to the redox behavior of humic acid
Schmeide, K.; Sachs, S.; Bernhard, G.;
A TRLFS study of curium(III) naphthalene and hydroxyquinoline complexes in aqueous solution
Moll, H.; Bernhard, G.;
TRLFS study on the speciation of uranium in seepage water and pore water of heavy metal contaminated soil
Baumann, N.; Arnold, T.; Lonschinski, M.;
Eukaryotic life in Biofilms formed in a Uranium Mine
Zirnstein, I.; Arnold, T.; Krawczyk-Bärsch, E.; Jenk, U.; Bernhard, G.; Röske, I.;
Aqueous uranyl benzoate species characterized by different spectroscopic techniques
Frost, L.; Moll, H.; Bernhard, G.;

Contact

Dr. Johannes Raff
Head Biogeochemistry
j.raffAthzdr.de
Phone: +49 351 260 2951
Fax: +49 351 260 12951