Multiplexed nanowire-based field effect transistors for immunosensing
The ultimate aim is to develop the nanosensory microsystem for multiparametric modular monitoring of immunotherapeutic processes, possessing the self-regulation functions. We establish a new immuno-chip platform based on an array of field effect transistor devices for (a) time lapse measurements and (b) specific analysis of T cell / tumor cell interactions, i.e. via biomolecular sensing of the inverse concentration of the tumor binding domain or anti-inflammatory cytokines.
In this context we exemplarily analyse the interaction between antigens and antigen-targeting molecules as well as the interaction between peptides and peptide-binding domains.
- Baraban, Larysa & Ibarlucea, Bergoi & Baek, Eunhye & Cuniberti, Gianaurelio. (2019). Hybrid Silicon Nanowire Devices and Their Functional Diversity. Advanced Science. 6. 10.1002/advs.201900522.
- Ibarlucea, Bergoi & Akbar, Teuku & Kim, Kihyun & Rim, Taiuk & Baek, Chang-Ki & Ascoli, Alon & Tetzlaff, Ronald & Baraban, Larysa & Cuniberti, Gianaurelio. (2017). Ultrasensitive detection of Ebola matrix protein in a memristor mode. Nano Research. 1-12. 10.1007/s12274-017-1720-2.
Impedance nanocytometer for label-free peripheral blood testing
The primary goal of the current research line is to explore the electrical properties of living cells in dynamic sensing and demonstrate a combination of the ultracompact detection element (around only 40µm2) with the machine learning concept to interpret the measured data. Nanoscopic gold nanowire electrodes, integrated into a microfluidic channel, act as nanocapacitors and measure in time domain the change of the amplitude and phase of the output voltage reflecting the electrical properties of living cells. In this respect we constructed an ultra-compact nanosized flow cytometer for real-time impedimetric detection and classification of subpopulations of living cells even in a label free manner.
- Schütt, Julian & Sandoval Bojorquez, Diana & Avitabile, Elisabetta & Mata, Eduardo & MIlyukov, Gleb & Colditz, Juliane & Delogu, Lucia & Rauner, Martina & Feldmann, Anja & Koristka, Stephanie & Middeke, Jan & Sockel, Katja & Fassbender, Juergen & Bachmann, Michael & Bornhauser, Martin & Cuniberti, Gianaurelio & Baraban, Larysa. (2020). Nanocytometer for smart analysis of peripheral blood and acute myeloid leukemia: a pilot study. Nano Letters. XXXX. 10.1021/acs.nanolett.0c02300.
High throughput characterization of kinetics of biological and chemical processes, including tracking the proliferation of living cells or monitoring enzymatic activity, is a key task, particularly, in medical diagnostics, food processing and pharmaceutical industries. We propose the new format of the in vitro assay using nano- to femto-liter emulsion droplets in fluidics that helps to surpass the precision of conventional tests. In particular, the approach can be used to determine e.g. metabolic activity, division rate and drug sensitivity in fundamental microbiology research and clinical studies.
- Baraban, Larysa & Bertholle, Fabien & Salverda, Merijn & Bremond, Nicolas & Panizza, Pascal & Baudry, Jean & de Visser, J Arjan & Bibette, Jérôme. (2011). Millifluidic droplet analyser for microbiology. Lab on a chip. 11. 4057-62. 10.1039/c1lc20545e.
- Illing, Rico & Burkart, Corinna & Pfitzner, Daniel & Jungmann, Dirk & Baraban, Larysa & Cuniberti, Gianaurelio. (2016). Ecotoxicity assessment using ciliate cells in millifluidic droplets. Biomicrofluidics. 10. 024115. 10.1063/1.4944869.