Vitae of Astrid Barkleit

Scientific career

• since 2000:
  Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, scientific staff member
• 2007-2014:
  Technische Universität Dresden, Radiochemistry Division, research associate
• 2000-2004:
  Universität Rostock, postgraduate studies in Environmental Protection, Diploma in Life Sciences
• 1999-2000:
  Leibniz Institute for New Materials Saarbrücken, research associate
• 1999:
  Universität des Saarlandes Saarbrücken, Institute of Inorganic Chemistry, PhD thesis: "Synthesis and Investigations of Structure and Reactivity of Metalchelatkomplexes of Tridentate Disilazane Ligands"
• 1996-1998:
  Universität des Saarlandes, Institute of Inorganic Chemistry, research associate
• 1989-1996:
  Georg-August-Universität Göttingen, Universität des Saarlandes, Diploma in Chemistry

Research experience

• Actinide speciation in biosystems and in aqueous solutions, namely U(VI), Cm(III), Am(III) and Eu(III)
• Application of various spectroscopic techniques, in particular time-resolved laser-induced fluorescence spectroscopy (TRLFS)
• Decommissioning of nuclear facilities: Determination of activities in components of nuclear power plants

Current projects

• Joint project EBENE - Experimentally supported calculations of neutron fields and the resulting activities in spaces far from the reactor (BMBF, FORKA, 15S9447A, 2024-2027).

• Joint project RADEKOR - Speciation and transfer of radionuclides in the human organism especially taking into account decorporation agents.
  Project coordinator (BMBF, 02NUK057A, 2020-2024).

Past projects

• Joint project EMPRADO - Development of a method for pre-activity and dose rate calculations of components in the reactor vicinity based on neutron fluence distributions (BMBF, FORKA, 15S9409A, 2018-2024).
• WERREBA - Means for efficient decommissioning of reactor components and concrete shielding: Calculation of the activity inventory and their validation on drilling cores as well as mobility investigations of radionuclides
  (BMBF, FORKA, 15S9412, 2019-2022).

• EU project INSIDER - Improved nuclear site characterization for waste minimization in decommissioning and dismantling operations under constrained environment (Horizon 2020 EURATOM, 2017-2021).

• Joint project TransAqua - Transfer of radionuclides in aquatic ecosystems. Subproject Biokinetic models: "Spectroscopic determination of the binding form (speciation) of trivalent actinides and lanthanides in biofluids of the human intestinal tract and in the blood" (KVSF, BMBF, 02NUK030F, 2012-2017)
• Joint Project: Retention of repository-relevant radionuclides in natural clay rocks and in saline systems. Subproject: "Thermodynamics of the interaction of An(III)/Ln(III) with borate and organic repository-relevant ligands" (BMWi, 02E11021, 2011-2014)
• Joint Project: Interaction and transport of actinides in natural clay rock with consideration of humic substances and clay organics. Subproject: "Investigations of temperature dependence of complexation and sorption of trivalent actinides in the system actinid-NOM-natural clay rock-aquifer" (BMWi, 02E10417, 2007-2010)
• Binding form of Cm(III) and Eu(III) in human body fluids (saliva, urine) (DFG, BE2234/10-1/2, 2007-2010)
• The mobilization of actinides by microbial ligands taking into consideration the final storage of nuclear waste. Interactions of selected actinides with pyoverdins and related model compounds. (BMBF, 02E9985, 2005-2007)

Publications

2024

• Senwitz, C., Butscher, D., Holtmann, L., Vogel, M., Steudtner, R., Drobot, B., Stumpf, T., Barkleit, A., Heller, A.: Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro. Sci. Total Environ., 293, 171374 (2024)

2023

• Sucena, S.F.; Demirer, T.I.; Baitullina, A.; Hagenbach, A.; Grewe, J.; Spreckelmeyer, S.; März, J.; Barkleit, A.; Maia, P.I.d.S.; Nguyen, H.H.; Abram, U.: "Gold-Based Coronands as Hosts for M³⁺ Metal Ions: Ring Size Matters." Molecules, 28, 5421 (2023).
• Friedrich, S.; Sieber, C.; Drobot, B.; Tsushima, S.; Barkleit, A.; Schmeide, K.; Stumpf, T.; Kretzschmar, J.: "Eu(III) and Cm(III) Complexation by the Aminocarboxylates NTA, EDTA, and EGTA Studied with NMR, TRLFS, and ITC—An Improved Approach to More Robust Thermodynamics." Molecules, 28, 4881 (2023).

2021

• Heller, A.; Pisarevskaja, A.; Bölicke, N.; Barkleit, A.; Bok, F.; Wober, J.: "The effect of four lanthanides onto a rat kidney cell line (NRK-52E) is dependent on the composition of the cell culture medium." Toxicol. 456, 152771 (2021)
• Moll, H.; Barkleit, A.; Frost, L.; Raff, J.: "Curium(III) speciation in the presence of microbial cell wall components." Ecotoxicol. Environ. Saf., 227, 112887 (2021).

2020

• Tamain, C.; Bonato, L.; Aupiais, J.; Dumas, T.; Guillaumont, D.; Barkleit, A.; Berthon, C.; Solari, P. L.; Ikeda-Ohno, A.; Guilbaud, P.; Moisy, P.: "Role of the hydroxo group in the coordination of citric acid toward trivalent americium." Eur. J. Inorg. Chem., 1331-1344 (2020)
• Kretzschmar, J.; Strobel, A.; Haubitz, T.; Steudtner, R.; Barkleit, A.; Brendler, V.; Stumpf, T.: "Uranium(VI) complexes of glutathione disulfide forming in aqueous solution."  Inorg. Chem., 59, 4244-4254 (2020)
• Höllriegl, V.; Barkleit, A.; Spielmann, V.; Li, W. B.: "Measurement, model prediction and uncertainty quantification of plasma clearance of cerium citrate in humans." Rad. Environ. Biophys., 59, 121-130 (2020)

2019

• H. Zänker, K. Heine, S. Weiss, V. Brendler, R. Husar, G. Bernhard, K. Gloe, T. Henle, A. Barkleit: "Strong Uranium(VI) Binding onto Bovine Milk Proteins, Selected Protein Sequences and Model Peptides." Inorg. Chem., 58, 4173-4189 (2019)
•  A. Heller, A. Barkleit, F. Bok, J. Wober: "Effect of four lanthanides onto the viability of two mammalian kidney cell lines." Ecotoxicol. Environ. Saf., 173, 469-481 (2019)
•  A. Rossberg, T. Abe, K. Okuwaki, A. Barkleit, K. Fukuzawa, T. Nakano, Y. Mochizuki, S. Tsushima: "Destabilization of DNA through interstrand crosslinking by UO₂²⁺." Chem. Commun., 55, 2015-2018 (2019)

2018

• A. Barkleit, C. Hennig, A. Ikeda-Ohno: "Interaction of Uranium(VI) with α‑Amylase and Its Implication for Enzyme Activity." Chem. Res. Toxicol., 31, 1032-1041 (2018).

2017

• P. Baumgart, A. Eltner, A.R. Domula, A. Barkleit, D. Faust: "Scale dependent soil erosion dynamics in a fragile loess landscape." Zeitschrift für Geomorphologie, 61(3), 191-206 (2017).
• C. Wilke, A. Barkleit, T. Stumpf, A. Ikeda-Ohno: "Speciation of the trivalent f-elements Eu(III) and Cm(III) in digestive media." J. Inorg. Biochem., 175, 248-258 (2017).
• A. Barkleit, C. Wilke, A. Heller, T. Stumpf, A. Ikeda-Ohno: "Trivalent f-elements in human saliva: A comprehensive speciation study by time-resolved laser-induced fluorescence spectroscopy and thermodynamic calculations." Dalton Trans., 46, 1593-1605 (2017).

2016

• S.E. Hellebrandt, S. Hofmann, N. Jordan, A. Barkleit, M. Schmidt: "Incorporation of Eu(III) into Calcite under Recrystallization conditions." Scientific Reports, 6, 33137 (2016).
• A. Barkleit, A. Heller, A. Ikeda-Ohno, G. Bernhard: "Interaction of europium and curium with alpha-amylase." Dalton Trans., 45, 8724-8733 (2016).

2015

• J. Schott, J. Kretzschmar, S. Tsushima, B. Drobot, M. Acker, A. Barkleit, S. Taut, V. Brendler, T. Stumpf: "The interaction of Eu(III) with organoborates - a further approach to understand the complexation in the An/Ln(III)-borate system." Dalton Trans. 44(24), 11095-11108 (2015).
• U. Weinert, K. Pollmann, A. Barkleit, M. Vogel, T. Günther, J. Raff: "Synthesis of S-layer conjugates and evaluation of their modifiability as a tool for the functionalization and patterning of technical surfaces." Molecules 20, 9847-9861 (2015).
• A.A.A. Osman, G. Geipel, A. Barkleit, G. Bernhard: "Uranium(VI) binding forms in selected human body fluids: thermodynamic calculations versus spectroscopic measurements." Chem. Res. Toxicol. 28(2), 238-247 (2015).
• T. Reitz, A. Rossberg, A. Barkleit, R. Steudtner, S. Selenska-Pobell, M.L. Merroun: "Spectroscopic study on uranyl carboxylate complexes formed at the surface layer of Sulfolobus acidocaldarius." Dalton Trans. 44(6), 2684-2692 (2015).

2014

• T. Reitz, A. Rossberg, A. Barkleit, S. Selenska-Pobell, M.L. Merroun: "Decrease of U(VI) immobilization capability of the facultative aerobic strain Paenibacillus sp. JG-TB8 under anoxic conditions due to strongly reduced phosphatase activity." PLOS One 9(8), e102447. (2014).
• L. Opherden, J. Oertel, A. Barkleit, K. Fahmy, A. Keller: "Paramagnetic Decoration of DNA Origami Nanostructures by Eu³⁺ Coordination." Langmuir 30, 8152-8159 (2014).
• A. Sabau, Y. Pipon, N. Toulhoat, C. Lomenech, N. Jordan, N. Moncoffre, A. Barkleit, N. Marmier, V. Brendler, S. Surblé, E. Giffaut: "Interaction of europium and nickel with calcite studied by Rutherford Backscattering Spectrometry and Time-Resolved Laser Fluorescence Spectroscopy." Nucl. Instrum. Meth. B 332, 111-116 (2014).
• J. Schott, J. Kretzschmar, M. Acker, S. Eidner, M. Kumke, B. Drobot, A. Barkleit, S. Taut, V. Brendler, T. Stumpf: "Formation of a Eu(III) borate solid species from a weak Eu(III) borate complex in aqueous solution." Dalton Trans. 43 (30), 11516-11528 (2014).
• A. Barkleit, J. Kretzschmar, S. Tsushima, M. Acker: "Americium(III) and europium(III) complex formation with lactate at elevated temperatures studied by spectroscopy and quantum chemical calculations." Dalton Trans. 43 (29), 11221-11232 (2014).

2013

• B. Raditzky, S. Sachs, K. Schmeide, A. Barkleit, G. Geipel, G. Bernhard: "Spectroscopic study of americium(III) complexes with nitrogen containing organic model ligands." Polyhedron 65, 244-251 (2013).
• A. Barkleit, M. Acker, G. Bernhard: "Europium(III) complexation with salicylic acid at elevated temperatures." Inorg. Chim. Acta 394, 535-541 (2013).
• H. Moll, L. Luetke, A. Barkleit, G. Bernhard: "Curium(III) Speciation Studies with Cells of a Groundwater Strain of Pseudomonas fluorescens." Geomicrobiol. J. 30, 337-346 (2013).

2012

• A. Heller, A. Barkleit, H. Foerstendorf, S. Tsushima, K. Heim, G. Bernhard: "Curium(III) citrate speciation in biological systems: a europium(III) assisted spectroscopic and quantum chemical study." Dalton Trans. 41, 13969-13983 (2012).
• J. Schott, M. Acker, A. Barkleit, V. Brendler, S. Taut, G. Bernhard: "The influence of temperature and small organic ligands on the sorption of Eu(III) on Opalinus Clay." Radiochim. Acta 100, 315-324 (2012).

2011

• A. Barkleit, H. Foerstendorf, B. Li, A. Rossberg, H. Moll, G. Bernhard: "Coordination of uranium(VI) with functional groups of bacterial lipopolysaccharide studied by EXAFS and FT-IR spectroscopy." Dalton Trans. 38, 9868-9876 (2011).
• A. Barkleit, S. Tsushima, O. Savchuk, J. Philipp, K. Heim, M. Acker, S. Taut, K. Fahmy: "Eu³⁺-Mediated Polymerization of Benzenetetracarboxylic Acid Studied by Spectroscopy, Temperature-Dependent Calorimetry, and Density Functional Theory." Inorg. Chem. 50, 5451-5459 (2011).
• A. Heller, A. Barkleit, G. Bernhard: "Chemical speciation of trivalent actinides and lanthanides in biological fluids: The dominant in vitro binding form of curium(III) and europium(III) in human urine." Chem. Res. Toxicol. 24, 193-203 (2011).
• A. Barkleit, G. Geipel, M. Acker, S. Taut, G. Bernhard: "First fluorescence spectroscopic investigation of Am(III) complexation with an organic carboxylic ligand, pyromellitic acid." Spectrochim. Acta A: Mol. Biomol. Spectrosc. 78, 549-552 (2011).

2010

• A. Heller, O. Rönitz, A. Barkleit, G. Bernhard, J.-U. Ackermann: "Complexation of Europium(III) with the Zwitterionic Form of Amino Acids Studied with Ultraviolet–Visible and Time-Resolved Laser-Induced Fluorescence Spectroscopy." Appl. Spectrosc. 64, 930-935 (2010).
• B. Li, J. Raff, A. Barkleit, G. Bernhard, H. Foerstendorf: "Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach." J. Inorg. Biochem. 7, 718-725 (2010).

2009

• A. Barkleit, H. Moll, G. Bernhard: "Complexation of uranium(VI) with peptidoglycan." Dalton Trans. 27, 5379-5385 (2009).
• A. Heller, A. Barkleit, G. Bernhard, J.U. Ackermann: "Complexation study of europium(III) and curium(III) with urea in aqueous solution investigated by time-resolved laser-induced fluorescence spectroscopy." Inorg. Chim. Acta 362, 1215-1222 (2009).

2008

• A. Barkleit, H. Moll, G. Bernhard: "Interaction of uranium(VI) with lipopolysaccharide." Dalton Trans. 2879-2886 (2008).
• A. Barkleit, H. Foerstendorf, K. Heim, S. Sachs, G. Bernhard: "Complex formation of uranium(VI) with l-phenylalanine and 3-phenylpropionic acid studied by attenuated total reflection fourier transform infrared spectroscopy." Appl. Spectrosc. 62, 798-802 (2008).

2007

• A. Koban, G. Bernhard: "Uranium(VI) complexes with phospholipid model compounds – A laser spectroscopic study." J. Inorg. Biochem. 101, 750-757 (2007).

before 2007

• A. Koban, G. Geipel, A. Roßberg, G. Bernhard: "Uranium(VI) complexes with sugar phosphates in aqueous solution." Radiochim. Acta 92, 903-908 (2004).
• A. Koban, G. Bernhard: "Complexation of uranium(VI) with glycerol 1-phosphate." Polyhedron 23, 1793-1797 (2004).
• A. Koban, G. Geipel, G. Bernhard: "Complex formation between uranium(VI) and α-D-glucose-1-phosphate." Radiochim. Acta 91, 393-396 (2003).
• M. Veith, A. Koban, V. Huch: "Ring opening of thiophene: Synthesis and crystal structure of bis(diethyl ether)bis(µ₄-{(3,4-η)-4-[1,1,3,3-tetramethyl-3-(2-thienyl)disilazan-1-yl-κN:κN]but-l-en-3-yne-1-thiolato(2-)-κS:κS:κS})tetralithium ([(C₄H₃S)SiMe₂‑N(Li)‑SiMe₂‑C≡C‑CH=CH‑SLi·OEt₂]₂)." Helv. Chim. Acta 81, 1640-1644 (1998).
• M. Veith, A. Koban, K. Fries, P. Spaniol, R. Elsässer, A. Rammo, V. Huch, U. Kleinsteuber: "Lithium coordination in chelating silazanes of the general formula [X‑Me₂Si‑N‑SiMe₂‑X]₂Li₂." Organometallics 17, 2612-2618 (1998).