Lecture: Biophysics (TU Dresden)

The lecture is part of the Master curriculum „Physics of Life“ at the Technische Universität Dresden and held during the winter semester as a two weeks course, followed by two weeks of practicals in labs at the HZDR and the university. The lecture covers state of the art methods that address the structural and physical properties of biopolymers. Spectroscopic methods such as Infrared-, Raman-, Circular Dichroism and Fluorescence-Spectroscopy are introduced, both in static and time-resolved applications. Corresponding experiments are carried out in the practical courses. The lecture is further accompanied by student presentations of scientific papers.

Part I

• Electromagnetic waves and UV-vis Absoprtion
• Detectors and Gratings
• Time-correlated single photon counting

Part II

• Spectroscopic analyses of protein secondary structure
• Circular Dichroism
• Infrared and Raman Spectroscopy

Part III

• Fluorescence spectroscopy
• Vibronic coupling
• Fluorescence detection of protein unfolding and ligand binding