Biosorption of heavy metals and nanoparticles by Gram-positive bacteria and cellular components


Biosorption of heavy metals and nanoparticles by Gram-positive bacteria and cellular components

Suhr, M.; Matys, S.; Viacava Romo, K. E.; Raff, J.; Pollmann, K.

Bacterial isolates from the uranium mining waste pile Haberland (Johanngeorgenstadt, Saxony) possess high affinities to heavy metals e.g. uranium. This binding effect is caused by the components of the bacterial cell wall, mainly affected by surface layer proteins.
In this work we studied the metal uptake of different environmental relevant heavy and noble metals and metaloids as well as nanoparticles by living bacterial cells of a Gram-positive strain and its isolated primary cellular components (e.g. membrane lipids, peptidoglycan, s-layer proteins). This was done via batch experiments and the results were obtained by ICP-MS measurements.
The quartz crystal microbalance with dissipation monitoring was introduced to track the adsorption of biological molecules and to study the metal and nanoparticle interaction with the single cell wall components. With this method it was able to detect the sorption processes on a molecular level in real time and to obtain further information to metal interaction processes and to viscoelastic properties. Partially supporting atomic force microscopy (AFM) studies enable the imaging of bio nanostructures and reveal complex information of structural properties.

Keywords: Biosorption; bacteria; QCM-D; ICP-MS; s-layer; proteins; lipids; heavy metals; nanoparticles

  • Poster
    8th HZDR PhD Seminar, 07.-09.10.2013, Bautzen, Germany

Permalink: https://www.hzdr.de/publications/Publ-19237
Publ.-Id: 19237