Amphiphilic polymer coating, radiolabel functionalization and in vitro behaviour of ultrasmall iron oxide nanoparticles in different tumour cell lines


Amphiphilic polymer coating, radiolabel functionalization and in vitro behaviour of ultrasmall iron oxide nanoparticles in different tumour cell lines

Pombo Garcia, K.; Zarschler, K.; Bergmann, R.; Steinbach, J.; Stephan, H.; Barreto, J. A.; Spiccia, L.; Graham, B.

There is currently great interest in the application of nanoparticles for cancer imaging [1]. Due to their small size, biocompatibility and unique magnetic properties, ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) are one of the most attractive candidates as advanced biomedical materials [2]. A number of different methods have been used to transfer USPIONs into aqueous solution, one of the most successful of which has been the embedding of an amphiphilic polymer within the hydrophobic coating of the as-synthesized particles [3]. This strategy also provides a ready route to further chemical modification and functionalization of the outer surface with different molecules such as radiolabels, which may be used to help understand the biodistribution of the nanoparticles in vivo [4, 5].
We report the synthesis of functionalized USPIONs with a 5 nm core and stabilized by octylamine-modified polyacrylic acid (OPA) for potential use in magnetic resonance imaging (MRI). To assess their in vitro and in vivo behaviour, a 64Cu(II) chelator, N-(4-aminophenyl)-2-[4,7-bis(2-pyridylmethyl)-1,4,7-triazacyclononan-1-yl]acetamide (ami-no-dmptacn) was conjugated to the OPA chains for radioactivity-based detection and the nanoparticles tested on a range of different tumor cell lines (FaDu, MDA-MB435S, A431). Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) confirmed that the OPA-USPIONs are relatively monodisperse and stable in aqueous media. Temperature-dependent cellular uptake experiments indicate that the OPA-USPIONs can be internalized into cells via an energy-dependent cellular pathway, however no evidence of cytotoxicity is observed upon incubating the particles with the three different tumor cell lines for up to 72 h.
References:
[1] J. A. Barreto et al., Adv. Mater. 2011, 23, H18.
[2] R. Costo et. al., Langmuir 2011, 28, 178.
[3] A. Quarta et al., Nanoscale 2012, 4, 3319.

  • Poster
    GDCh Wissenschaftsforum, 01.-04.09.2013, Darmstadt, Deutschland

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Publ.-Id: 21388