Phage surface display derived short peptides for the development of new biosorbents for the recovery of valuable elements from primary and secondary sources


Phage surface display derived short peptides for the development of new biosorbents for the recovery of valuable elements from primary and secondary sources

Matys, S.; Lederer, F.; Schönberger, N.; Braun, R.; Pollmann, K.

In the course of evolution, nature has developed diverse strategies to avoid toxic effects of metals in the surrounding environment. Many organisms are able to bind metals to their surface via a variety of structurally diverse biomolecules carrying different functional groups. Because of the need for green and sustainable economic solutions, innovative biotechnological processes using biomolecules have become increasingly important.Biomolecules as sorbents are not only attractive for bioremediation, but also for the recovery of elements from recycling or mining. Currently, we are focusing on the development of new biosorbents based on short peptides for the selective recovery of valuable or toxic elements in complex process water streams such as Co, Ni, Ga or As and microparticles containing rare earth elements from fluorescent lamp powder. To select and identify these peptides we recently established Phage Surface Display Technology (PSD) as novel biotechnological platform in our group. Different adapted experimental setups for the peptide selection of each respective target material could be established and will be presented in detail. Specific and selectively metal binding peptides for particulate materials as well as for ionic species could be identified from commercially available phage libraries. These libraries contain a pool of genetically engineered phage of up to 10^9 different peptide motifs presented at their surface, which will be reduced in variability in an iterative biopanning process to capture the best binders. This contribution will give an insight into current research activities in our group focusing on PSD, introducing suitable spectroscopic techniques to characterize the metal-peptide complexes and discuss strategies for technical applications.

Keywords: peptides; metal binding; phage surface display; metal peptide complexes

  • Lecture (Conference)
    Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie, 15.-18.04.2018, Wolfsburg, Deutschland

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Publ.-Id: 26703