Bioassociation of U(VI) and Eu(III) by plant (Brassica napus) suspension cell cultures – A spectroscopic investigation


Bioassociation of U(VI) and Eu(III) by plant (Brassica napus) suspension cell cultures – A spectroscopic investigation

Jessat, J.; Sachs, S.; Moll, H.; John, W.; Steudtner, R.; Hübner, R.; Bok, F.; Stumpf, T.

In this study, we investigated the interaction of U(VI) and Eu(III) with Brassica napus suspension plant cells as a model system. Concentration-dependent (0-200 µM) bioassociation experiments showed that more than 75% of U(VI) and Eu(III) were immobilized by the cells. In addition to this phenomenon, time-dependent studies for 1 to 72 h of exposure showed a multi-stage bioassociation process for cells that were exposed to 200 µM U(VI), where, after initial immobilization of U(VI) within 1 h of exposure, it was released back into culture medium starting within 24 h. A re-mobilization to this extent has not been previously observed. The MTT assay was used to correlate the bioassociation behavior of Eu and U with the cell vitality. Speciation studies by spectroscopy and in silico methods highlighted various U and Eu species over the course of exposure. We were able to observe a new U species, which emerged simultaneously with the re-mobilization of U back into solution, which we assume to be a U(VI) phosphate species. Thus, the interaction of U(VI) and Eu(III) with released plant metabolites could be concluded.

Keywords: plant cells; bioassociation; Brassica napus; time-resolved laser-induced fluorescence spectroscopy; radionuclides; uranium; europium; speciation

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Publ.-Id: 31335