Identification of cancer cells by a combination of FTIR spectroscopy and PET


Identification of cancer cells by a combination of FTIR spectroscopy and PET

Steiner, G.; Richter, T.; Salzer, R.; Bergmann, R.; Rodig, H.; Johannsen, B.

A combination of FTIR spectroscopy and positron emission tomography (PET) is shown to provide new information on tissue. Here we give a first demonstration on the potential of this combination in discriminating tumor tissue from healthy tissue. Examples are taken of cancer grown in muscle tissue in mice. Immediately before thin sections of the cancer tissue were prepared, a radiotracer was injected in the living mouse. Subsequently a native section was immobilized on a CaF2 window and an autoradiographic image was recorded from that immobilized section. FTIR maps of the thin sections were obtained by using an infrared microscope equipped with computerized XY stage and MCT detector. Principal component analysis was chosen for chemometric evaluation of the spectra. Evaluated data were reassembled into two dimensional maps and compared with the corresponding PET image.

Keywords: FTIR spectroscopy; FTIR mapping; cancer; tumor; autoradiography; radiotracer; positron emission tomography; principal component analysis

  • Lecture (Conference)
    SPIE, Photonics West 2000, "Spectral Imaging: Instrumentation, Applications and Analysis" (3920) San Jose, CA, USA, 22.1.2000 - 28.1.2000
  • Contribution to external collection
    SPIE, Photonics West 2000, "Spectral Imaging: Instrumentation, Applications and Analysis" (3920) San Jose, CA, USA, 22.1.2000 - 28.1.2000

Permalink: https://www.hzdr.de/publications/Publ-3160
Publ.-Id: 3160