Screening Arrays of Laminin Peptides on Modified Cellulose for Promotion of Adhesion of Primary Endothelial and Neural Precursor Cells


Screening Arrays of Laminin Peptides on Modified Cellulose for Promotion of Adhesion of Primary Endothelial and Neural Precursor Cells

Wetzel, R.; Hauser, S.; Lin, W.; Berg, P.; Werner, C.; Pietzsch, J.; Kempermann, G.; Zhang, Y.

Neural precursor cells (NPC) are primary cells intensively used in the context of research on adult neurogenesis and modelling of neuronal development in health and diseased states. Substrates that can facilitate NPC adhesion will be very useful for culturing these cells. Due to the presence of laminin in basal lamina as well as their involvement in differentiation, migration, and adhesion of many types of cells, we focused on surfaces modified with laminin-derived peptides and compared them with the widely used fibronectin-derived RGD peptides. We synthesized an array of 46 peptides on cellulose paper (SPOT) to identify laminin-derived peptides that promote short-term adhesion of murine NPC and human primary endothelial cells. Various previously reported peptide sequences have been re-evaluated in this work. Initial adhesion experiments showed NPC preferred several laminin-derived peptides by up to 5-time higher cell numbers, compared to the well-known promiscuous integrin binding RGD peptide. Importantly, screening of cell adhesion has revealed a synergetic effect of filamentous matrix, peptide sequence, surface property, ligand density, and the dynamic process of NPC adhesion.

Keywords: peptide array; SPOT synthesis; laminin peptides; neural precursor cells; endothelial cells

Permalink: https://www.hzdr.de/publications/Publ-31762
Publ.-Id: 31762