Interactions of the S-Layer protein of the uranium waste pile isolate bacillus sphaericus JG-A 12 with metals


Interactions of the S-Layer protein of the uranium waste pile isolate bacillus sphaericus JG-A 12 with metals

Raff, J.; Wahl, R.; Matys, S.; Flemming, K.; Mertig, M.; Selenska-Pobell, S.; Pompe, W.

Bacteria in uranium waste piles are of special interest for bioremediation, because they are adapted to high concentrations of radionuclides and heavy metals which occur in these environments. A large number of Bacillus strains was recovered from a uranium mining waste pile "Haberland" near the town of Johanngeorgenstadt in Saxony, Germany. One isolate, Bacillus sphaericus JG-A 12, possesses a surface layer protein with a molecular weight of 135 kDa [1]. Sequence analysis of a part of the gene of this protein, coding 184 amino acids from the N-terminal region of its matured S-layer product, demonstrated that the protein shares only a limited homol-ogy (less than 35%) to the known S-layers of the other Bacillus sphaericus strains WHO 2362, CCM 2177 and P-1 (see Fig. 1).

  • Poster
    BMRI-2 Euroconference on Bacterial-Metal/Radionuclide Interaction, Rossendorf bei Dresden, 30.8.-1.9. 2000

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Publ.-Id: 3480