Bacterial diversity in waters at the Siberian deep-well monitoring site Tomsk-7


Bacterial diversity in waters at the Siberian deep-well monitoring site Tomsk-7

Nedelkova, M.; Selenska-Pobell, S.

Bacterial diversity was studied in water samples collected at depths of 290 to 324 m from the Siberian deep-well radioactive waste disposal site S15 near the Russian city of Tomsk by using the 16S rDNA retrieval. Bacterial clone libraries were constructed for the DNA isolated from the biomass collected on filters with a pore size of 0.45µm and 0.22µm. Comparative analyses of the two libraries revealed significant differences in the predominant bacterial groups and in the species composition. In the 0.45µm clone library a dominance of Dechlorosoma spp. was found, whereas in the 0.22µm library populations of Chrysobacterium spp. belonging to Cytophaga/Flavobacterium/ Bacteroides (CFB) group were predominant. Many of the identified 16S rDNA sequences were affiliated to uncultured members of different bacterial lineages. Effort to culture some of them is in progress in our laboratory.
In addition, using CFB-specific primers and applying semi-nested PCR a clone library was generated for the total DNA extracted from the same site. Our previous study of this DNA, applying universal bacterial primers, indicated a presence of relatively small CFB population there. The estimation of the real amount and the significance of this bacterial group via real-time PCR is also in progress.

VAAM-2004 vom 28.03-31.03.04 in Braunschweig

  • Poster
    VAAM-2004 vom 28.03-31.03.04 in Braunschweig
  • Contribution to proceedings
    VAAM 2004, 28.-31.03.2004, Braunschweig, Germany
    Proceedings
  • Contribution to external collection
    Tsang, C. F.; Apps., J. A.: Developments in Water Science, Underground Injection Science and Technology, Amsterdam: Elsevier, 2005, 0-444-52068-6, 521-536

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Publ.-Id: 6024