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1 PublicationDNA Based Formation of Nanodisc-Stacks
Subramanian, M.; Kielar, C.; Tsushima, S.; Fahmy, K.; Oertel, J.
We describe here the formation of multimers of membrane-scaffolding protein MSP1D1-bounded nanodiscs using the thiol reactivity of engineered cysteines. The mutated positions N42 and K163 in MSP1D1 were chosen to support chemical modification as evidenced by fluorescent labeling with pyrene. The direct disulphide bond formation of nanodiscs formed by the MSP1D1_N42C variant led to dimers and trimers with low yield. In contrast, transmission electron microscopy revealed that the attachment of oligonucleotides to the engineered cysteines of MSP1D1 allowed the growth of submicron-sized tracts of stacked nanodiscs through the hybridization of nanodisc populations carrying complementary strands and a flexible spacer.
Keywords: membrane-scaffolding protein; nanodisc; membrane protein; lipid bilayer; lipid protein interaction; multimerization; self-assembly; bionanotechnology
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- DOI: 10.3390/molecules26061647 references this (Id 32927) publication
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DNA-Mediated Stack Formation of Nanodiscs
ROBIS: 32443 has used this (Id 32927) publication of HZDR-primary research data
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Reseach data in the HZDR data repository RODARE
Publication date: 2021-12-14 Closed access
DOI: 10.14278/rodare.1321
Versions: 10.14278/rodare.1322
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Permalink: https://www.hzdr.de/publications/Publ-32927