Classification of Desulfovibrio isolates recovered from a uranium mining weaste pile

A large variety of bacteria was demonstrated to be present in soil and sediment samples of a uranium waste pile in Saxony, Germany. Anaerobic sulfate-reducing bacteria belonging to the genus Desulfovibrio were found among them. The study of these bacteria, which are also known to reduce U(VI), is of great importance for the development of bioremediation procedures for decontamination of the environments polluted with uranium. The indigenous for the waste Desulfovibrio isolates were classified by the use of Amplified Ribosomal DNA Restriction Endonucleases Analysis (ARDREA), Random Amplified Polymorphic DNA (RAPD), Repetitive Primer Amplified Polymorphic DNA (rep-APD), 16S rDNA Sequencing, and as well as with classical microbiological methods.
Using 16S- and IGS-ARDREA the pile isolates were phylogenetically affiliated to Desulfovibrio vulgaris (oxamicus). The RAPD and rep-APD analyses have demonstrated a close genomic relationship between the pile isolates, but D. vulgaris (oxamicus) 1925T was not closely related to them. These results are in agreement to the taxonomic characterization of the strains by comparison of the fatty acid spectra.