Coomassie-Brilliant Blue Staining of Polyacrylamide Gels


Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

Arndt, C.; Koristka, S.; Feldmann, A.; Bergmann, R.; Bachmann, M.

Over the past a series of staining procedures for proteins were published. Still, the most commonly used staining dye for proteins is Coomassie-Brilliant Blue. The major reasons are: Coomassie-Brilliant Blue staining is simple, fast and sensitive. As Coomassie-Brilliant Blue is almost insoluble in water a series of procedures including colloidal aqueous procedures were described.

Keywords: Coomassie Brilliant Blue; Polyacrylamide gels; Proteins

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