Isolation of Proteins from Polyacrylamide Gels

Isolation of Proteins from Polyacrylamide Gels

Koristka, S.; Arndt, C.; Bergmann, R.; Bachmann, M.

Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.

Keywords: Polyacrylamide gels; Isolation of proteins

  • Book chapter
    Kurien B., Scofield R.: Electrophoretic Separation of Proteins. Methods in Molecular Biology, Totowa, NJ, USA: Human Press, 2019, 461-465
    DOI: 10.1007/978-1-4939-8793-1_38

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