Helmholtz-Zentrum Dresden-Rossendorf |
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Biogeochemistry |
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Dr. Johannes Raff
Head Biogeochemistry |
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| Phone: | +49 351 260 2951 | ||
| Email: | j.raff hzdr.de | ||
| Link: | ORCID: 0000-0002-0520-3611 | ||
| Address | Bautzner Landstraße 400 01328 Dresden | ||
| Building/Office: | 801/P314 | ||
Scientific career
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Since 2017:Head of the Department Biogeochemistry - Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
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Since 2016:Guest lecturer at Univerity Granada, Spain
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Since 2015:Guest lecturer at Technische Universität Dresden
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Since 2012:Assistant lecturer at University of Applied Sciences, Dresden
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2002-2017:Research Assistant - Institute of Resource Ecology and since 2011 additionally at Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
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1997-2002:PhD student at University Leipzig/Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
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1990-1996:University Bayreuth, Diploma in Biology
Research work
Investigation of the interaction of radionuclides and industrial metals with bioligands and microorganisms. The aim of the work is to elucidate molecular processes in nature and on the cellular level and to understand their interaction. The results obtained serve on one hand to improve risk assessment and on the other hand for the development of new materials and processes for the detection, removal and recovery of metals.
Projects
- BioVeStRa, support code 02S9276A, duration 01.06.2016-31.05.2019
- BioNEWS, support code 03WKCL03F, duration 01.01.2015-30.12.2017
- S-Sieve, support code 490 ZBG, duration 01.06.2013-29.02.2016
- Technology transfer project BIOBASE, support code 325/20517289/IMA, duration 01.09.2012-31.03.2013
- HEF-BioBase, support code HE-2012-04, duration 01.07.2012-30.06.2013
- Textilien mit S-Layer, support code VF110032, duration 01.06.2012-30.09.2014
- NanoPharm, support code 03X0094G, duration 01.06.2010-30.09.2013
- ForMaT: BioNano, support code 03FO3141, duration 28.09.2009-31.03.2010
- BIOREM, support code 03EGSSN014, duration 01.04.2008-31.03.2009
- Urantransport, support code 02S8517, duration 01.10.2007-30.09.2010
- NanoFoto, support code 01SF0717, duration 01.10.2007-31.12.2008
Publications
2020
Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips
Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Blaschke, D.; Schmidt, H.
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Biosensors 10(2020), 7
DOI: 10.3390/bios10010007
Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12
Moll, H.; Lehmann, F.; Raff, J.
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Colloids and Surfaces B: Biointerfaces 190(2020), 110950
DOI: 10.1016/j.colsurfb.2020.110950
2019
P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications
Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Schmidt, H.
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Biosensors 9(2019)4, 120
DOI: 10.3390/bios9040120
How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?
Kumar, S.; Creff, G.; Hennig, C.; Rossberg, A.; Steudtner, R.; Raff, J.; Vidaud, C.; Oberhaensli, F. R.; Bottein, Y.; Den Auwer, C.
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Chemistry - A European Journal 25(2019), 12332-12341
DOI: 10.1002/chem.201902015
Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin
Drobot, B.; Schmidt, M.; Mochizuki, Y.; Abe, T.; Okuwaki, K.; Brulfert, F.; Falke, S.; Samsonov, S.; Komeiji, Y.; Betzel, C.; Stumpf, T.; Raff, J.; Tsushima, S.
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Physical Chemistry Chemical Physics 21(2019)38, 21213-21222
DOI: 10.1039/C9CP03750K
S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water
Jung, J.; Lakatos, M.; Bengs, S.; Matys, S.; Raff, J.; Blüher, A.; Cuniberti, G.
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Colloids and Surfaces B: Biointerfaces 183(2019), 110284
DOI: 10.1016/j.colsurfb.2019.06.014
2018
Bio-recycling of metals: Recycling of technical products using biological applications
Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.
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Biotechnology Advances 36(2018)4, 1048-1062
DOI: 10.1016/j.biotechadv.2018.03.006
2017
Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling
Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.
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Poster
22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland -
Contribution to proceedings
22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland -
Solid State Phenomena 262(2017), 389-393
DOI: 10.4028/www.scientific.net/SSP.262.389
Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus
Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.
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European Biophysics Journal 46(2017)1, 77-89
DOI: 10.1007/s00249-016-1139-9
2016
Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources
Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.
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Minerals 6(2016)2, 54
DOI: 10.3390/min6020054
Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins
Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.
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PLOS ONE (2016), 0156785
DOI: 10.1371/journal.pone.0156785
Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes
Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.
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Analytical Chemistry 88(2016)7, 3548-3555
DOI: 10.1021/acs.analchem.5b03958
Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies
Suhr, M.; Raff, J.; Pollmann, K.
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Journal of Visualized Experiments 107(2016), e53572
DOI: 10.3791/53572
2015
Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate
Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.
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Inorganic Chemistry 54(2015)14, 6758-6762
DOI: 10.1021/acs.inorgchem.5b00555
Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces
Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.
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Molecules 20(2015), 9847-9861
DOI: 10.3390/molecules20069847
Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis
Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.
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Chemical Science 6(2015), 964-972
DOI: 10.1039/C4SC02022G
S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces
Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.
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Engineering in Life Sciences 15(2015)7, 710-720
DOI: 10.1002/elsc.201400204
Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM
Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.
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BioMetals 27(2015)6
DOI: 10.1007/s10534-014-9794-8
Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles
Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.
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Talanta 144(2015), 241-246
DOI: 10.1016/j.talanta.2015.05.082
2014
Immobilization of microorganisms for AFM studies in liquids
Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.
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RSC Advances 4(2014), 51156-51164
DOI: 10.1039/C4RA03874F
Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie
Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.
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Biospektrum 20(2014)2, 172-175
DOI: 10.1007/s12268-014-0425-2
Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP
Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.
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RSC Advances 4(2014), 19970-19979
DOI: 10.1039/C4RA02928C
Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods
Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.
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BioMetals 27(2014)4, 775-785
DOI: 10.1007/s10534-014-9772-1
S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability
Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.
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Journal of Photochemistry and Photobiology A: Chemistry 277(2014), 12-18
DOI: 10.1016/j.jphotochem.2013.12.004
2013
Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes
Weinert, U.; Pollmann, K.; Raff, J.
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Sensors and Actuators B 185(2013), 553-559
DOI: 10.1016/j.snb.2013.05.051
Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53
Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.
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Microbiology 159(2013), 1097-1108
DOI: 10.1099/mic.0.065763-0
2012
Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells
Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
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Microbial Cell Factories 11(2012), 163
DOI: 10.1186/1475-2859-11-163
2011
E. coli filament formation induced by heterologous S-layer expression
Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.
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Bioengineered Bugs 2(2011)3, 178-181
DOI: 10.4161/bbug.2.3.15418
2010
Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity
Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.
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Science of the Total Environment 409(2010)2, 384-395
DOI: doi:10.1016/j.scitotenv.2010.10.011
Self assembling proteins as technology platform for the development of new bio-composite materials
Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
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Lecture (Conference)
12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland - Materialwissenschaft und Werkstofftechnik (2010)
Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach
Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.
- Journal of Inorganic Biochemistry 104(2010)7, 718-725
Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane
Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.
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Microbiology 156(2010), 3584-3595
DOI: 10.1099/mic.0.040808-0
2008
Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris
Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.
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BioMetals 21(2008)3, 333-341
DOI: 10.1007/s10534-007-9122-7
2007
Magnetic properties of transition-metal nanoclusters on a biological substrate
Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.
- Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
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Lecture (Conference)
International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan
2006
Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy
Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.
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Biophysical Journal 91(2006)3, 996-1007
DOI: 10.1529/biophysj.105.079137
Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells
Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.
- Letters in Applied Microbiology 43(2006)1, 39-45
Metal binding by bacteria from uranium mining waste piles and its technological applications
Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.
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Biotechnology Advances 24(2006)1, 58-68
DOI: 10.1016/j.biotechadv.2005.06.002
2005
Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements
Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.
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Microbiology 151(2005)9, 2961-2973
DOI: 10.1099/mic.0.28201-0
Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12
Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
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Applied and Environmental Microbiology 71(2005)9, 5532-5543
DOI: 10.1128/AEM.71.9.5532-5543.2005
2004
Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers
Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.
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Chemistry of Materials 16(2004)26, 5549-5551
DOI: 10.1021/cm040191v
Bacteria from extreme environments: basis for new technical applications
Raff, J.; Selenska-Pobell, S.
- BioMeT-Magazin (2004), 18
Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies
Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
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Lecture (Conference)
14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark - Geochimica et Cosmochimica Acta 68(2004)11, 499-499
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Contribution to proceedings
14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark
Proceedings
Use of Bacteria for Cleaning Radioactive Wastewaters
Raff, J.; Selenska-Pobell, S.
- Genomics/Proteomics Technology 22(2004)2, 32L-32N
2003
Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers
Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.
- Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)
Biosorption of Uranium and Copper by Biocers
Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.
- Chemistry of Materials, 15 (1), 240-244, Jan. 2003
2001
A fast screening method for surface layers on Gram-positive bacteria
Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.
- Biotechnology letters 23, 1485-1490 (2001)
Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602
Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.
- Advanced Materials 2001, 13, No. 10, May 17, p. 736-740
EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces
Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.
- Radiochimica Acta 89 (2001), 625-631
2000
Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile
Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.
- Radiochimica Acta 88, 71-76 (2000)
