Helmholtz-Zentrum Dresden-Rossendorf
Biogeochemistry
	Dr. Johannes Raff Head Biogeochemistry
	Phone:	+49 351 260 2951
	Email:	j.raffhzdr.de
	Link:	ORCID: 0000-0002-0520-3611
	Address	Bautzner Landstraße 400 01328 Dresden
	Building/Office:	801/P314

Scientific career

Since 2017:

Head of the Department Biogeochemistry - Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
Since 2016:

Guest lecturer at Univerity Granada, Spain
Since 2015:

Guest lecturer at Technische Universität Dresden
Since 2012:

Assistant lecturer at University of Applied Sciences, Dresden
2002-2017:

Research Assistant - Institute of Resource Ecology and since 2011 additionally at Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
1997-2002:

PhD student at University Leipzig/Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e.V.
1990-1996:

University Bayreuth, Diploma in Biology

Research work

Investigation of the interaction of radionuclides and industrial metals with bioligands and microorganisms. The aim of the work is to elucidate molecular processes in nature and on the cellular level and to understand their interaction. The results obtained serve on one hand to improve risk assessment and on the other hand for the development of new materials and processes for the detection, removal and recovery of metals.

Projects

BioVeStRa, support code 02S9276A, duration 01.06.2016-31.05.2019
BioNEWS, support code 03WKCL03F, duration 01.01.2015-30.12.2017
S-Sieve, support code 490 ZBG, duration 01.06.2013-29.02.2016
Technology transfer project BIOBASE, support code 325/20517289/IMA, duration 01.09.2012-31.03.2013
HEF-BioBase, support code HE-2012-04, duration 01.07.2012-30.06.2013
Textilien mit S-Layer, support code VF110032, duration 01.06.2012-30.09.2014
NanoPharm, support code 03X0094G, duration 01.06.2010-30.09.2013
ForMaT: BioNano, support code 03FO3141, duration 28.09.2009-31.03.2010
BIOREM, support code 03EGSSN014, duration 01.04.2008-31.03.2009
Urantransport, support code 02S8517, duration 01.10.2007-30.09.2010
NanoFoto, support code 01SF0717, duration 01.10.2007-31.12.2008

Publications

2020

Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Blaschke, D.; Schmidt, H.

Biosensors 10(2020), 7
DOI: 10.3390/bios10010007

Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12

Moll, H.; Lehmann, F.; Raff, J.

Colloids and Surfaces B: Biointerfaces 190(2020), 110950
DOI: 10.1016/j.colsurfb.2020.110950

2019

P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Schmidt, H.

Biosensors 9(2019)4, 120
DOI: 10.3390/bios9040120

How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?

Kumar, S.; Creff, G.; Hennig, C.; Rossberg, A.; Steudtner, R.; Raff, J.; Vidaud, C.; Oberhaensli, F. R.; Bottein, Y.; Den Auwer, C.

Chemistry - A European Journal 25(2019), 12332-12341
DOI: 10.1002/chem.201902015

Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin

Drobot, B.; Schmidt, M.; Mochizuki, Y.; Abe, T.; Okuwaki, K.; Brulfert, F.; Falke, S.; Samsonov, S.; Komeiji, Y.; Betzel, C.; Stumpf, T.; Raff, J.; Tsushima, S.

Physical Chemistry Chemical Physics 21(2019)38, 21213-21222
DOI: 10.1039/C9CP03750K

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Jung, J.; Lakatos, M.; Bengs, S.; Matys, S.; Raff, J.; Blüher, A.; Cuniberti, G.

Colloids and Surfaces B: Biointerfaces 183(2019), 110284
DOI: 10.1016/j.colsurfb.2019.06.014

2018

Bio-recycling of metals: Recycling of technical products using biological applications

Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.

Biotechnology Advances 36(2018)4, 1048-1062
DOI: 10.1016/j.biotechadv.2018.03.006

2017

Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling

Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.

Poster
22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
Contribution to proceedings
22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
Solid State Phenomena 262(2017), 389-393
DOI: 10.4028/www.scientific.net/SSP.262.389

Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus

Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.

European Biophysics Journal 46(2017)1, 77-89
DOI: 10.1007/s00249-016-1139-9

2016

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.

Minerals 6(2016)2, 54
DOI: 10.3390/min6020054

Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins

Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.

PLOS ONE (2016), 0156785
DOI: 10.1371/journal.pone.0156785

Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes

Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.

Analytical Chemistry 88(2016)7, 3548-3555
DOI: 10.1021/acs.analchem.5b03958

Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies

Suhr, M.; Raff, J.; Pollmann, K.

Journal of Visualized Experiments 107(2016), e53572
DOI: 10.3791/53572

2015

Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate

Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.

Inorganic Chemistry 54(2015)14, 6758-6762
DOI: 10.1021/acs.inorgchem.5b00555

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.

Molecules 20(2015), 9847-9861
DOI: 10.3390/molecules20069847

Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis

Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.

Chemical Science 6(2015), 964-972
DOI: 10.1039/C4SC02022G

S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces

Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.

Engineering in Life Sciences 15(2015)7, 710-720
DOI: 10.1002/elsc.201400204

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM

Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.

BioMetals 27(2015)6
DOI: 10.1007/s10534-014-9794-8

Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles

Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.

Talanta 144(2015), 241-246
DOI: 10.1016/j.talanta.2015.05.082

2014

Immobilization of microorganisms for AFM studies in liquids

Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.

RSC Advances 4(2014), 51156-51164
DOI: 10.1039/C4RA03874F

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.

Biospektrum 20(2014)2, 172-175
DOI: 10.1007/s12268-014-0425-2

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.

RSC Advances 4(2014), 19970-19979
DOI: 10.1039/C4RA02928C

Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.

BioMetals 27(2014)4, 775-785
DOI: 10.1007/s10534-014-9772-1

S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability

Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.

Journal of Photochemistry and Photobiology A: Chemistry 277(2014), 12-18
DOI: 10.1016/j.jphotochem.2013.12.004

2013

Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes

Weinert, U.; Pollmann, K.; Raff, J.

Sensors and Actuators B 185(2013), 553-559
DOI: 10.1016/j.snb.2013.05.051

Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.

Microbiology 159(2013), 1097-1108
DOI: 10.1099/mic.0.065763-0

2012

Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells

Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.

Microbial Cell Factories 11(2012), 163
DOI: 10.1186/1475-2859-11-163

2011

E. coli filament formation induced by heterologous S-layer expression

Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.

Bioengineered Bugs 2(2011)3, 178-181
DOI: 10.4161/bbug.2.3.15418

2010

Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.

Science of the Total Environment 409(2010)2, 384-395
DOI: doi:10.1016/j.scitotenv.2010.10.011

Self assembling proteins as technology platform for the development of new bio-composite materials

Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.

Lecture (Conference)
12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland
Materialwissenschaft und Werkstofftechnik (2010)

Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach

Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.

Journal of Inorganic Biochemistry 104(2010)7, 718-725

Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane

Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.

Microbiology 156(2010), 3584-3595
DOI: 10.1099/mic.0.040808-0

2008

Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris

Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.

BioMetals 21(2008)3, 333-341
DOI: 10.1007/s10534-007-9122-7

2007

Magnetic properties of transition-metal nanoclusters on a biological substrate

Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.

Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
Lecture (Conference)
International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan

2006

Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy

Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.

Biophysical Journal 91(2006)3, 996-1007
DOI: 10.1529/biophysj.105.079137

Toxic avengers

Raff, J.; Selenska-Pobell, S.

Nuclear Engineering International 51(2006)619, 34-36

Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells

Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.

Letters in Applied Microbiology 43(2006)1, 39-45

Metal binding by bacteria from uranium mining waste piles and its technological applications

Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.

Biotechnology Advances 24(2006)1, 58-68
DOI: 10.1016/j.biotechadv.2005.06.002

2005

Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements

Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.

Microbiology 151(2005)9, 2961-2973
DOI: 10.1099/mic.0.28201-0

Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.

Applied and Environmental Microbiology 71(2005)9, 5532-5543
DOI: 10.1128/AEM.71.9.5532-5543.2005

2004

Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers

Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.

Chemistry of Materials 16(2004)26, 5549-5551
DOI: 10.1021/cm040191v

Bacteria from extreme environments: basis for new technical applications

Raff, J.; Selenska-Pobell, S.

BioMeT-Magazin (2004), 18

Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.

Lecture (Conference)
14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark
Geochimica et Cosmochimica Acta 68(2004)11, 499-499
Contribution to proceedings
14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark
Proceedings

Use of Bacteria for Cleaning Radioactive Wastewaters

Raff, J.; Selenska-Pobell, S.

Genomics/Proteomics Technology 22(2004)2, 32L-32N

2003

Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers

Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.

Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)

Biosorption of Uranium and Copper by Biocers

Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.

Chemistry of Materials, 15 (1), 240-244, Jan. 2003

2001

A fast screening method for surface layers on Gram-positive bacteria

Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.

Biotechnology letters 23, 1485-1490 (2001)

Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602

Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.

Advanced Materials 2001, 13, No. 10, May 17, p. 736-740

EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces

Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.

Radiochimica Acta 89 (2001), 625-631

2000

Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile

Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.

Radiochimica Acta 88, 71-76 (2000)

Helmholtz-Zentrum Dresden-Rossendorf

Biogeochemistry

Scientific career

Research work

Projects

Publications

2020

Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips

Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12

2019

P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications

How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?

Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

2018

Bio-recycling of metals: Recycling of technical products using biological applications

2017

Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling

Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus

2016

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins

Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes

Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies

2015

Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis

S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM

Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles

2014

Immobilization of microorganisms for AFM studies in liquids

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability

2013

Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes

Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

2012

Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells

2011

E. coli filament formation induced by heterologous S-layer expression

2010

Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Self assembling proteins as technology platform for the development of new bio-composite materials

Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach

Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane

2008

Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris

2007

Magnetic properties of transition-metal nanoclusters on a biological substrate

2006

Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy

Toxic avengers

Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells

Metal binding by bacteria from uranium mining waste piles and its technological applications

2005

Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements

Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12

2004

Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers

Bacteria from extreme environments: basis for new technical applications

Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies

Use of Bacteria for Cleaning Radioactive Wastewaters

2003

Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers

Biosorption of Uranium and Copper by Biocers

2001

A fast screening method for surface layers on Gram-positive bacteria

Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602

EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces

2000

Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie