Helmholtz-Zentrum Dresden-Rossendorf


Dr. Johannes Raff

Head Biogeochemistry


+49 351 260 2951




ORCID: 0000-0002-0520-3611


Bautzner Landstraße 400 - 01328 Dresden

Scientific career

  • Since 2018:
    Head of the Department Biogeochemistry - Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e. V.
  • Since 2016:
    Guest lecturer at Univerity Granada, Spain
  • Since 2015:
    Guest lecturer at Technische Universität Dresden
  • Since 2012:
    Assistant lecturer at University of Applied Sciences, Dresden
  • 2002-2018:
    Research Assistant - Institute of Resource Ecology and since 2011 additionally at Helmholtz Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf e. V.
  • 1997-2002:
    PhD student at University Leipzig/Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf e. V.
  • 1990-1996:
    University Bayreuth, Diploma in Biology

Research work

Investigation of the interaction of radionuclides and industrial metals with bioligands and microorganisms. The aim of the work is to elucidate molecular processes in nature and on the cellular level and to understand their interaction. The results obtained serve on one hand to improve risk assessment and on the other hand for the development of new materials and processes for the detection, removal and recovery of metals.


  • RENA, support code 02NUK066A, duration 01.09.2021-31.08.2024
  • BioVeStRa, support code 02S9276A, duration 01.06.2016-31.05.2019
  • BioNEWS, support code 03WKCL03F, duration 01.01.2015-30.12.2017
  • S-Sieve, support code 490 ZBG, duration 01.06.2013-29.02.2016
  • Technology transfer project BIOBASE, support code 325/20517289/IMA, duration 01.09.2012-31.03.2013
  • HEF-BioBase, support code HE-2012-04, duration 01.07.2012-30.06.2013
  • Textilien mit S-Layer, support code VF110032, duration 01.06.2012-30.09.2014
  • NanoPharm, support code 03X0094G, duration 01.06.2010-30.09.2013
  • ForMaT: BioNano, support code 03FO3141, duration 28.09.2009-31.03.2010
  • BIOREM, support code 03EGSSN014, duration 01.04.2008-31.03.2009
  • Urantransport, support code 02S8517, duration 01.10.2007-30.09.2010
  • NanoFoto, support code 01SF0717, duration 01.10.2007-31.12.2008



Biostimulation of Indigenous Microbes for Uranium Bioremediation in Former U Mine Water: Multidisciplinary approach assessment

Newman Portela, A.; Krawczyk-Bärsch, E.; Lopez Fernandez, M.; Bok, F.; Kassahun, A.; Drobot, B.; Steudtner, R.; Stumpf, T.; Raff, J.; Merroun, M. L.


Uranium(VI) interactions with Pseudomonas sp. PS-0-L, V4-5-SB and T5-6-I

Kasko, J.; Li, X.; Müller, K.; Ge, Y.; Vettese, G. F.; Law, G. T. W.; Siitari-Kauppi, M.; Huittinen, N. M.; Raff, J.; Bomberg, M.; Herzig, M.

How tobacco (Nicotiana tabacum) BY-2 cells cope with Eu(III) – A microspectroscopic study.

Klotzsche, M.; Vogel, M.; Sachs, S.; Raff, J.; Stumpf, T.; Drobot, B.; Steudtner, R.


Speciation and spatial distribution of Eu(III) in fungal mycelium

Günther, A.; Wollenberg, A.; Vogel, M.; Drobot, B.; Steudtner, R.; Freitag, L.; Hübner, R.; Stumpf, T.; Raff, J.

Peptidoglycan as major binding motif for Uranium bioassociation on Magnetospirillum magneticum AMB-1 in contaminated waters

Krawczyk-Bärsch, E.; Ramtke, J.; Drobot, B.; Müller, K.; Steudtner, R.; Kluge, S.; Hübner, R.; Raff, J.


Curium(III) speciation in the presence of microbial cell wall components

Moll, H.; Barkleit, A.; Frost, L.; Raff, J.

Detecting bacterial cell viability in few μl solutions from impedance measurements on silicon‐based biochips

Bhat, V. J.; Vegesna, S. V.; Kiani, M.; Zhao, X.; Blaschke, D.; Du, N.; Vogel, M.; Kluge, S.; Raff, J.; Hubner, U.; Skorupa, I.; Rebohle, L.; Schmidt, H.

Involved research facilities

Related publications

Spatially resolved Eu(III) environments by chemical microscopy

Vogel, M.; Steudtner, R.; Fankhänel, T.; Raff, J.; Drobot, B.

Related publications

Uranium(VI) bioassociation by different fungi – a comparative study into molecular processes

Wollenberg, A.; Drobot, B.; Hübner, R.; Kretzschmar, J.; Freitag, L.; Lehmann, F.; Günther, A.; Stumpf, T.; Raff, J.

Involved research facilities

Related publications


Survival of the basidiomycete Schizophyllum commune in soil under hostile environmental conditions in the Chernobyl Exclusion Zone

Traxler, L.; Wollenberg, A.; Steinhauser, G.; Chyzhevskyi, I.; Dubchak, S.; Großmann, S.; Günther, A.; Gupta, D. K.; Iwannek, K.-H.; Kirieiev, S.; Lehmann, F.; Schulz, W.; Walther, C.; Raff, J.; Kothe, E.

Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Blaschke, D.; Schmidt, H.

Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12

Moll, H.; Lehmann, F.; Raff, J.


P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Schmidt, H.

Involved research facilities

Related publications

How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?

Kumar, S.; Creff, G.; Hennig, C.; Rossberg, A.; Steudtner, R.; Raff, J.; Vidaud, C.; Oberhaensli, F. R.; Bottein, Y.; Den Auwer, C.

Involved research facilities

Related publications

Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin

Drobot, B.; Schmidt, M.; Mochizuki, Y.; Abe, T.; Okuwaki, K.; Brulfert, F.; Falke, S.; Samsonov, S.; Komeiji, Y.; Betzel, C.; Stumpf, T.; Raff, J.; Tsushima, S.

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Jung, J.; Lakatos, M.; Bengs, S.; Matys, S.; Raff, J.; Blüher, A.; Cuniberti, G.


Bio-recycling of metals: Recycling of technical products using biological applications

Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.


Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling

Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.

  • Poster
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Contribution to proceedings
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Open Access Logo Solid State Phenomena 262(2017), 389-393
    DOI: 10.4028/www.scientific.net/SSP.262.389
    ISSN: 1662-9779
    Cited 3 times in Scopus

Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus

Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.


Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.

Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins

Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.

Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes

Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.

Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies

Suhr, M.; Raff, J.; Pollmann, K.


Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate

Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.

Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis

Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.

S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces

Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM

Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.

Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles

Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.


Immobilization of microorganisms for AFM studies in liquids

Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.

Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.

Involved research facilities

Related publications

S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability

Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.


Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes

Weinert, U.; Pollmann, K.; Raff, J.

Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.


Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells

Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.


E. coli filament formation induced by heterologous S-layer expression

Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.


Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.

Involved research facilities

Related publications

Self assembling proteins as technology platform for the development of new bio-composite materials

Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.

  • Lecture (Conference)
    12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland
  • Materialwissenschaft und Werkstofftechnik (2010)

Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach

Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.

  • Journal of Inorganic Biochemistry 104(2010)7, 718-725

Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane

Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.


Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris

Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.


Magnetic properties of transition-metal nanoclusters on a biological substrate

Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.

  • Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
  • Lecture (Conference)
    International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan


Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy

Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.

Toxic avengers

Raff, J.; Selenska-Pobell, S.

  • Nuclear Engineering International 51(2006)619, 34-36

Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells

Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.

  • Letters in Applied Microbiology 43(2006)1, 39-45

Metal binding by bacteria from uranium mining waste piles and its technological applications

Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.


Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements

Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.

Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.


Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers

Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.

Bacteria from extreme environments: basis for new technical applications

Raff, J.; Selenska-Pobell, S.

  • BioMeT-Magazin (2004), 18

Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.

  • Lecture (Conference)
    14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark
  • Geochimica et Cosmochimica Acta 68(2004)11, 499-499
    ISSN: 0016-7037
  • Contribution to proceedings
    14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark

Use of Bacteria for Cleaning Radioactive Wastewaters

Raff, J.; Selenska-Pobell, S.

  • Genomics/Proteomics Technology 22(2004)2, 32L-32N


Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers

Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.

  • Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)

Biosorption of Uranium and Copper by Biocers

Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.

  • Chemistry of Materials, 15 (1), 240-244, Jan. 2003


A fast screening method for surface layers on Gram-positive bacteria

Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.

  • Biotechnology letters 23, 1485-1490 (2001)

Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602

Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.

  • Advanced Materials 2001, 13, No. 10, May 17, p. 736-740

EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces

Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.

  • Radiochimica Acta 89 (2001), 625-631


Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile

Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.

  • Radiochimica Acta 88, 71-76 (2000)