Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf

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Development of metal ion binding peptides using phage surface display technology

Schönberger, N.; Matys, S.; Flemming, K.; Lehmann, F.; Lederer, F.; Pollmann, K.


Phage surface display technology is a useful tool for the identification of biosorptive peptides. In this work it is used for the identification of cobalt, nickel and gallium binding peptides. We present methods for the enrichment of metal ion binding bacteriophage clones from a commercial phage display library. Metal ion selective peptides are suitable to separate as well as concentrate cobalt and nickel from copper black shale leaching products (EcoMetals project) and gallium from industrial waste waters (EcoGaIn project). In contrast to common capture methods of specific binding phage for solid materials the ionic species have to be immobilized prior to the bio-panning procedure. This was realized by chemical complexation of the metal ions using commercial complexing agents on porous matrices. Moreover, an option to harvest non elutable strong binding phage is proposed.

Keywords: Phage Surface Display; biopanning; immobilized metal ions; nickel; gallium; cobalt; metal binding peptides

  • Contribution to proceedings
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
    22. International Biohydrometallurgy Symposium 2017


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