Department of Radiation research on biological systems
Why is research into ionizing radiation and the behavior of radionuclides in the environment still of central importance, even in the age of climate change and the energy transition?
Radionuclides are not only of natural origin but are also continuously released into the environment as a result of human activities. Despite decades of research, significant gaps in knowledge remain regarding the transport of radionuclides in natural systems, their uptake by organisms, and their long-term effects on ecosystems and humans. These questions are becoming increasingly important, particularly in light of the expansion of sustainable energy systems, the safe disposal of radioactive waste, and the sustainable protection of humans.
Against this backdrop, the “Radiation Research on Biological Systems” department investigates the influence of radioactive substances on biological and chemical processes. In doing so, it is important to develop a fundamental and molecular understanding of the transport behavior of radionuclides as well as their bioavailability, which govern the distribution and mobility of radionuclides in the biosphere. This enables a robust assessment of risks to humans and the environment while simultaneously opening up innovative applications. Our research encompasses the deep subsurface in the context of final disposal of high-level radioactive waste, environmental and industrial systems, as well as a wide variety of biological systems.
To this end, we combine modern approaches from molecular biology, microbiology, biochemistry, radioanalytics, spectroscopy, and microscopy. The insights gained contribute to the further development of transport and prediction models that take biological processes into account, support the development of sustainable remediation strategies for contaminated sites, and open up new perspectives for prevention research.
By understanding the complex interactions between biology, chemistry, and physics, we contribute to the safe use of nuclear technologies and the responsible handling of radioactive materials in a changing society.
Interested in working with us?
To support our work, we are always looking for students and interns from the fields of biology, chemistry and environmental sciences who either want to write their thesis with us or simply gain practical experience. Are you interested? Then please get in touch to clarify any questions you may have.
Current research topics
- Determination of the microbial diversity in water and soil environments contaminated with heavy metals and radionuclides as well as in different host rocks for potential radioactive waste disposals
- Microbe-radionuclide-interactions with reference strains and isolates
- Interaction of eukaryotic cells with radionuclides and lanthanides in particular with plants, fungi, sponges and algae
- Investigation of the interaction of selected bioligands and model compounds with f-elements
- Characterization of microbial processes affecting the conditions in deep geological repositories for radioactive waste (e.g. transformation of bentonite as barrier material)
- Calculation and determination of the metal speciation relevant to the environment
- Verification and validation of transport models
- Characterization of particles in the environment relevant to the transport of radionuclides
- Investigation of the transport processes of radionuclides by particles (including microbes) in natural water
Projects
- PIANOFORTE/EU project, grant number: 101061037, duration: 01.06.2022-31.05.2027
- EURAD2-InCoManD, EU-EURATOM, grant number: 10 45219 047, duration: 01.10.2024-30.09.2026
- Vernetzungsdoktoranden über die Helmholtz Forschungsfelder „Energie“ und „Gesundheit“ (zusammen mit dem Institut für Radioonkologie – OncoRay, Abteilung Strahlenbiologie, Prof. Nils Cordes): Alpha-Partikel: Identifizierung von Mechanismen von der Aufnahme bis zum Zelltod, Laufzeit: 01.12.2025 – 30.11.2028
- ReNATE: Biologische Radionuklidentfernung durch Nutzung natürlicher Assoziationsprozesse: Technische Entwicklung, BMFTR-Projekt, Förderkennzeichen: 15S9454A, Laufzeit: 01.10.2025 - 30.09.2028
An overview of completed projects can be found here.
Latest publication
Radiocopper in BCMA-targeted immunotheranostics of myeloma
Ullrich, M.; Zarschler, K.; Kubeil, M.; Laube, M.; Pietzsch, J.; Belter, B.
Abstract
Background: The theranostic potential of copper-64 (PET imaging) and copper-67 (SPECT imaging and therapy) is increasingly recognized. This work investigates the performance of this ‘true’ matched pair in a preclinical radioimmunotheranostic setting that utilizes experimental monoclonal antibodies (mAbs) directed against B cell maturation antigen (BCMA), a transmembrane glycoprotein that has emerged as a critical target for the treatment of multiple myeloma (MM).
Methods: The commercially available BCMA-directed mAbs MAB193 and Vicky 1 were modified with the bispidine N2py4 enabling chelation of radiocopper, and their binding affinity was determined using surface plasmon resonance and flow cytometry. Pharmacokinetics and tumor uptake of the copper 64-labeled mAbs were determined in mice bearing subcutaneous myeloma xenografts, using PET. The best-performing mAb was included in a pilot study on therapeutic use with copper-67 in U266 myeloma-bearing mice, involving dose monitoring and dose predictions for humans based on quantitative SPECT.
Results: After bioconjugation, both [64Cu]Cu N2py4-MAB193 and [64Cu]Cu N2py4-Vicky 1 maintained low nanomolar BCMA binding affinity. In PET imaging, [64Cu]Cu N2py4-MAB193 showed the highest uptake in U266 myeloma xenografts and the lowest normal tissue background, e.g., achieving a tumor-to-muscle contrast of 14.9 within 48 h. [67Cu]Cu N2py4-MAB193 delivered absorbed doses up to 1.26 Gy/MBq in U266 myeloma xenografts and reduced the tumor mass at total doses above 52 Gy. Predicted human effective doses were below 35.3 µSv/MBq for immunoPET and 162 µSv/MBq for radioimmunotherapy/immunoSPECT.
Conclusion: The fundamental efficacy of copper 64 and copper 67 in BCMA-targeted immunotheranostics of MM promises both precise PET-based dose planning as well as radioimmunotherapy in combination with highly sensitive SPECT-based dose monitoring, as demonstrated by the theranostic capabilities of [64Cu/67Cu]Cu N2py4-MAB193 in tumor-bearing mice. The results provide a strong incentive for incorporating the CopperNostics approach into the further development of BCMA-targeted radioimmunotheranostic agents, including precise tailoring of their pharmacokinetic properties to the physical half-life of copper-67.
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Theranostics 16(2026)15, 8461-8477
DOI: 10.7150/thno.134397
Permalink: https://www.hzdr.de/publications/Publ-43574
Team
Head | |||||
| Name | Bld./Office | +49 351 260 | |||
|---|---|---|---|---|---|
| Prof. Dr. Manja Kubeil | 801/P127 | 2006 | m.kubeil | ||
Employees | |||||
| Name | Bld./Office | +49 351 260 | |||
| Nicolas Tim Appel | 801/P306 | 2860 | n.appel | ||
Deep Biosphere | |||||
| Name | Bld./Office | +49 351 260 | |||
| Dr. Andrea Cherkouk | 801/P356 | 2989 | a.cherkouk | ||
| Dr. Stephan Hilpmann | 801/P318 | 2759 | s.hilpmann | ||
| Sindy Kluge | 801/P219 | 3194 | s.kluge | ||
| Katrin Rode | 801/P306 | 2860 | k.rode | ||
| Dr. Ting-Shyang Wei | t.wei | ||||
Terrestrial Microbiology | |||||
| Name | Bld./Office | +49 351 260 | |||
| Dr. Johannes Raff | 801/P314 | 2951 | j.raff | ||
| Rahel Bertheau | 801/P354 | 3138 | |||
| Dr. Alix Günther | 801/P256 | 2433 2522 | a.guenther | ||
| Dr. Evelyn Krawczyk-Bärsch | 801/P252 | 2076 | e.krawczyk-baersch | ||
Plants and Rhizosphere | |||||
| Name | Bld./Office | +49 351 260 | |||
| Dr. Susanne Sachs | 801/P208 | 2436 | s.sachs | ||
| Raul Eduardo Linares Jimenez | 801/P103 | 2457 | r.linares-jimenez | ||
| Julia Marie Mätzkow | 801/P153 | 3032 | j.maetzkow | ||
| Dr. Henry Moll | 801/P256 | 2433 2549 | h.moll | ||
| Jana Seibt | 801/P219 | 3194 2510 | j.seibt | ||
Spectroscopy and Speciation of f-elements | |||||
| Name | Bld./Office | +49 351 260 | |||
| Dr. Robin Steudtner | 801/P317 | 2895 | r.steudtner | ||
| Katrin Flemming | 801/P309 | 2958 | k.flemming | ||
