MICRONUC: MICRObial Activity in NUClear waste disposal
The safe storage of nuclear waste in deep geological repositories is a big challenge for our society. Comprehensive understanding of the migration of actinides within the respective host rock and the transport behavior after a possible release from the repository should is urgently needed. Detrimental effects of the radioactive waste on humans and the environment must be avoided over a very long-time period. Microorganisms, which exist in the host rock and backfill material, can survive under extreme conditions over a very long period of time. Their presence therein is of interest because microbial processes can affect the physical and geochemical conditions (e.g. pH, Eh, release of gases) on site. Through their activity, microorganisms can also influence other processes e.g. the corrosion of the container material and migration behavior of actinides, which is caused by the direct or indirect interaction of the microorganisms with the actinides. These processes depend on the type of microbial population, the actinide, and the geochemical circumstances.
The aim of this group is to study the microbial diversity and their activity in the potential host rocks (clay rock, salt rock, crystalline rock) and barrier materials (e.g. bentonite) for nuclear waste disposal and to evaluate their role in respect to the corrosion of container material and the migration of actinides in the geo- and biosphere.
Current research themes:
- Investigation of the microbial diversity in potential host rocks and barrier materials relevant for a nuclear waste repository in Germany by culture-independent (e.g. DNA/RNA extraction, 16S rRNA gene analysis,) as well as culture-dependent (enrichment and isolation of microorganisms) methods
- Micro- and mesocosm experiments to investigate the microbial activity in the barrier material depending on different repository relevant conditions and their effect on the corrosion of container material
- Characterization of the interaction mechanisms of microorganisms isolated from the different host rocks and barrier materials with actinides on a molecular level by applying a combination of different spectroscopies (e.g. Time-Resolved Laser-Induced Fluorescence Spectroscopy (TRLFS), Infrared Spectroscopy (IR) and X-ray absorption spectroscopy) and microscopic techniques such as Scanning Electron Microscopy (SEM) in combination with Energy-Dispersive X-ray spectroscopy (EDX).
- Use of meta-omics techniques to investigate metabolic networks and the influence of the biosphere on the migration behavior of radionuclides
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Bentonite alteration in batch reactor experiments with and without fertilizing agents: implications for the disposal of radioactive waste
Podlech, C.; Matschiavelli, N.; Peltz, M.; Kluge, S.; Arnold, T.; Cherkouk, A.; Meleshyn, A.; Grathoff, G.; Warr, L. N.
Minerals 11(2021), 932
Cited 6 times in Scopus
Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study
Pinel-Cabello, M.; Jroundi, F.; Lopez Fernandez, M.; Geffers, R.; Jarek, M.; Jauregui, R.; Link, A.; Vílchez-Vargas, R.; Merroun, M. L.
Journal of Hazardous Materials 405(2021), 123858
Cited 21 times in Scopus
Presence of Bradyrhizobium sp. under continental conditions in Central Europe
Griebsch, A.; Matschiavelli, N.; Lewandowska, S.; Schmidtke, K.
Agriculture 10(2020)10, 446
Cited 4 times in Scopus
Molecular binding of Eu(III)/Cm(III) by Stenotrophomonas bentonitica and its impact on the safety of future geodisposal of radioactive waste
Ruiz-Fresneda, M. A.; Lopez Fernandez, M.; Martinez-Moreno, M. F.; Cherkouk, A.; Ju-Nam, Y.; Ojeda, J. J.; Moll, H.; Merroun, M. L.
Environmental Science & Technology 54(2020), 15180-15190
Cited 8 times in Scopus
The year-long development of microorganisms in uncompacted Bavarian bentonite slurries at 30 °C and 60 °C
Matschiavelli, N.; Kluge, S.; Podlech, C.; Standhaft, D.; Grathoff, G.; Ikeda-Ohno, A.; Warr, L.; Chukharkina, A.; Arnold, T.; Cherkouk, A.
Environmental Science & Technology 53(2019)17, 10514-10524
Online First (2019) DOI: 10.1021/acs.est.9b02670
Cited 8 times in Scopus
Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon
Bader, M.; Moll, H.; Steudtner, R.; Lösch, H.; Drobot, B.; Stumpf, T.; Cherkouk, A.
Environmental Science and Pollution Research 26(2019), 9352-9364
Online First (2019) DOI: 10.1007/s11356-019-04165-7
Cited 9 times in Scopus
Microbial diversity in an arid, naturally saline environment
Bachran, M.; Kluge, S.; Lopez-Fernandez, M.; Cherkouk, A.
Microbial Ecology 78(2019)2, 494-505
Cited 20 times in Scopus
Impact of Haloarchaea on speciation of uranium – a multi-spectroscopic approach
Bader, M.; Rossberg, A.; Steudtner, R.; Drobot, B.; Großmann, K.; Schmidt, M.; Musat, N.; Stumpf, T.; Ikeda-Ohno, A.; Cherkouk, A.
- DOI: 10.1107/S1600577520014265 is cited by this (Id 28021) publication
Environmental Science and Technology 52(2018)21, 12895-12904
Cited 14 times in Scopus
Comparative analysis of uranium bioassociation with halophilic bacteria and archaea
Bader, M.; Müller, K.; Foerstendorf, H.; Schmidt, M.; Simmons, K.; Swanson, J. S.; Reed, D. T.; Stumpf, T.; Cherkouk, A.
PLOS ONE 13(2018)1, e0190953
Cited 20 times in Scopus