Publications Repository - Helmholtz-Zentrum Dresden-Rossendorf
1 PublicationCopper-mediated cross-linking of S100A4, but not of S100A2, results in proinflammatory effects in melanoma cells
Haase-Kohn, C.; Wolf, S.; Lenk, J.; Pietzsch, J.
Abstract
The aim of this study was to investigate the response to and the physiological consequences of copper-mediated cross-linking of S100A2 and S100A4, two members of the S100 family of EF-hand calcium-binding proteins. As demonstrated by electrophoresis and mass spectrometry techniques S100A2 and S100A4 show formation of cross-links due to copper-mediated oxidation of cysteine residues. For S100A4, but not for S100A2, this results in both increased activation of NFκB and secretion of TNF-α in human A375 and, to a higher extent, in RAGE-transfected melanoma cells. The data suggest that a prooxidative tumor microenvironment enhances proinflammatory and prometastatic action of S100A4.
Involved research facilities
- PET-Center
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Biochemical and Biophysical Research Communications 413(2011), 494-498
DOI: 10.1016/j.bbrc.2011.08.132
Cited 30 times in Scopus
Permalink: https://www.hzdr.de/publications/Publ-16203