Kontakt

Dr. Johannes Raff

Lei­ter Biogeochemie
j.raffAthzdr.de
Tel.: +49 351 260 2951

Lebenslauf Johannes Raff

Wissenschaftlicher Werdegang

  • Seit 2017:
    Leiter der Abteilung Biogeochemie am Institut für Ressourcenökologie, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • Seit 2016:
    Gastdozent an der Univerität Granada, Spanien
  • Seit 2015:
    Gastdozent an der Technischen Universität Dresden
  • Seit 2012:
    Lehrbeauftragter an der Hochschule für Technik und Wirtschaft Dresden
  • 2002-2017:
    Wissenschaftlicher Mitarbeiter am Institut für Ressourcenökologie und seit 2011 zusätzlich am Helmholtz-Institut Freiberg für Ressourcentechnologie, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1997-2002:
    Doktorand an der Universität Leipzig/Institut für Ressourcenökologie, Helmholtz-Zentrum Dresden-Rossendorf e.V.
  • 1990-1996:
    Universität Bayreuth, Diplom in Biologie

Forschungstätigkeit

Untersuchung der Wechselwirkung von Radionukliden und Industriemetallen mit Bioliganden und Mikroorganismen. Ziel der Arbeiten ist es, molekulare Prozesse in der Natur und auf Zellebene aufzuklären und ihr Zusammenwirken zu verstehen. Die erhaltenen Ergebnisse dienen einer verbesserten Risikoabschätzung und andererseits der Entwicklung von neuen Materialien und Verfahren für die Detektion, Entfernung und Gewinnung von Metallen.

Projekte

  • BioVeStRa, FKz 02S9276A, Laufzeit 01.06.2016-31.05.2019
  • BioNEWS, FKz 03WKCL03F, Laufzeit 01.01.2015-30.12.2017
  • S-Sieve, FKz 490 ZBG, Laufzeit 01.06.2013-29.02.2016
  • Ausgründungsprojekt BIOBASE, FKz 325/20517289/IMA, Laufzeit 01.09.2012-31.03.2013
  • HEF-BioBase, FKz HE-2012-04, Laufzeit 01.07.2012-30.06.2013
  • Textilien mit S-Layer, FKz VF110032, Laufzeit 01.06.2012-30.09.2014
  • NanoPharm, FKz 03X0094G, Laufzeit 01.06.2010-30.09.2013
  • ForMaT: BioNano, FKz 03FO3141, Laufzeit 28.09.2009-31.03.2010
  • BIOREM, FKz 03EGSSN014, Laufzeit 01.04.2008-31.03.2009
  • Urantransport, FKz 02S8517, Laufzeit 01.10.2007-30.09.2010
  • NanoFoto, FKz 01SF0717, Laufzeit 01.10.2007-31.12.2008

Veröffentlichungen

2020

Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Blaschke, D.; Schmidt, H.

Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12

Moll, H.; Lehmann, F.; Raff, J.

2019

P-N Junction-Based Si Biochips with Ring Electrodes for Novel Biosensing Applications

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Schmidt, H.

How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?

Kumar, S.; Creff, G.; Hennig, C.; Rossberg, A.; Steudtner, R.; Raff, J.; Vidaud, C.; Oberhaensli, F. R.; Bottein, Y.; Den Auwer, C.

Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin

Drobot, B.; Schmidt, M.; Mochizuki, Y.; Abe, T.; Okuwaki, K.; Brulfert, F.; Falke, S.; Samsonov, S.; Komeiji, Y.; Betzel, C.; Stumpf, T.; Raff, J.; Tsushima, S.

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Jung, J.; Lakatos, M.; Bengs, S.; Matys, S.; Raff, J.; Blüher, A.; Cuniberti, G.

2018

Bio-recycling of metals: Recycling of technical products using biological applications

Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.

2017

Use of specific metal binding of self-assembling S-layer proteins for metal bioremediation and recycling

Vogel, M.; Matys, S.; Lehmann, F.; Drobot, B.; Günther, T.; Pollmann, K.; Raff, J.
  • Poster
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Beitrag zu Proceedings
    22. International Biohydrometallurgy Symposium, 24.-27.09.2017, Freiberg, Deutschland
  • Open Access Logo Solid State Phenomena 262(2017), 389-393
    DOI: 10.4028/www.scientific.net/SSP.262.389

Analysis of self‑assembly of S‑layer protein slp‑B53 from Lysinibacillus sphaericus

Liu, J.; Falke, S.; Drobot, B.; Oberthuer, D.; Kikhney, A.; Guenther, T.; Fahmy, K.; Svergun, D.; Betzel, C.; Raff, J.

2016

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

Pollmann, K.; Kutschke, S.; Matys, M.; Kostudis, S.; Hopfe, S.; Raff, J.

Characterization of three different unusual S-layer proteins from Viridibacillus arvi JG-B58 that exhibits two super-imposed S-layer proteins

Suhr, M.; Lederer, F. L.; Guenther, T. J.; Raff, J.; Pollmann, K.

Speciation Studies of Metals in Trace Concentrations: The Mononuclear Uranyl(VI) Hydroxo Complexes

Drobot, B.; Bauer, A.; Steudtner, R.; Tsushima, S.; Bok, F.; Patzschke, M.; Raff, J.; Brendler, V.

Au-Interaction of Slp1 polymers and monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as tools for biomolecule-metal studies

Suhr, M.; Raff, J.; Pollmann, K.
  • Journal of Visualized Experiments 107(2016), e53572
    DOI: 10.3791/53572

2015

Nanopatterning of Magnetic CrNi Prussian Blue Nanoparticles Using a Bacterial S-layer as Biotemplate

Valero, E.; Martín, M.; Gálvez, N.; Sánchez, P.; Raff, J.; Merroun, M. L.; Dominguez-Vera, J. M.

Synthesis of S-Layer Conjugates and Evaluation of Their Modifiability as a Tool for the Functionalization and Patterning of Technical Surfaces

Weinert, U.; Pollmann, K.; Barkleit, A.; Vogel, M.; Günther, T.; Raff, J.

Combining Luminescence Spectroscopy, Parallel Factor Analysis and Quantum Chemistry to Reveal Metal Speciation - a Case Study of Uranyl (VI) Hydrolysis

Drobot, B.; Steudtner, R.; Raff, J.; Geipel, G.; Brendler, V.; Tsushima, S.

S-layer proteins as an immobilization matrix for aptamers on different sensor surfaces

Weinert, U.; Vogel, M.; Reinemann, C.; Strehlitz, B.; Pollmann, K.; Raff, J.

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53 – A combined study using QCM-D, ICP-MS and AFM

Suhr, M.; Unger, N.; Viacava, K. E.; Günther, T. J.; Raff, J.; Pollmann, K.

Colorimetric As (V) Detection Based on S-Layer Functionalized Gold Nanoparticles

Lakatos, M.; Matys, S.; Raff, J.; Pompe, W.

2014

Immobilization of microorganisms for AFM studies in liquids

Günther, T. J.; Suhr, M.; Raff, J.; Pollmann, K.

Microbial ecology: Characteristics of bacteria from heavy metal contaminated mining waste piles
Eigenschaften von Bakterien aus Schwermetall-kontaminierten Halden: Mikrobielle Ökologie

Lederer, F. L.; Günther, T. J.; Raff, J.; Flemming, K.; Pollmann, K.

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.

Interaction of U(VI) with Schizophyllum commune studied by microscopic and spectroscopic methods

Günther, A.; Raff, J.; Merroun, M. L.; Roßberg, A.; Kothe, E.; Bernhard, G.

S-layer proteins as possible immobilization matrix for photocatalysts – OH radical scavenging capacity and protein stability

Schmoock, C.; Börnick, H.; Vogel, M.; Lehmann, F.; Kutschke, S.; Raff, J.; Dittmar, T.; Worch, E.

2013

Fluorescence Resonance Energy Transfer by S-layer coupled fluorescence dyes

Weinert, U.; Pollmann, K.; Raff, J.

Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

Lederer, F.; Weinert, U.; Günther, T.; Raff, J.; Weiß, S.; Pollmann, K.

2012

Development of functionalised polyelectrolyte capsules using filamentous Escherichia coli cells

Lederer, F.; Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.

2011

E. coli filament formation induced by heterologous S-layer expression

Lederer, F.; Günther, T.; Raff, J.; Pollmann, K.

2010

Biosorption of U(VI) by the green algae Chlorella vulgaris in dependence of pH value and cell activity

Vogel, M.; Günther, A.; Rossberg, A.; Li, B.; Bernhard, G.; Raff, J.

Self assembling proteins as technology platform for the development of new bio-composite materials

Günther, T.; Weinert, U.; Raff, J.; Pollmann, K.
  • Vortrag (Konferenzbeitrag)
    12th International and Interdisciplinary Symposium Biomaterials and Biomechanics: Fundamentals and Clinical Applications 2010, 17.-19.03.2010, Essen, Deutschland
  • Materialwissenschaft und Werkstofftechnik (2010)

Complexation of U(VI) with highly phosphorylated protein, phosvitin. A vibrational spectroscopic approach

Li, B.; Barkleit, A.; Raff, J.; Bernhard, G.; Foerstendorf, H.
  • Journal of Inorganic Biochemistry 104(2010)7, 718-725

Heterologous expression of the S-layer-like protein SllB induces the formation of long filaments of E. coli consisting of protein stabilized outer membrane

Lederer, F.; Günther, T.; Flemming, K.; Raff, J.; Fahmy, K.; Springer, A.; Pollmann, K.

2008

Spectroscopic identifications of U(VI) species sorbed by the green algae Chlorella vulgaris

Günther, A.; Raff, J.; Geipel, G.; Bernhard, G.

2007

Magnetic properties of transition-metal nanoclusters on a biological substrate

Herrmannsdörfer, T.; Bianchi, A. D.; Papageorgiou, T. P.; Pobell, F.; Wosnitza, J.; Pollmann, K.; Merroun, M.; Raff, J.; Selenska-Pobell, S.
  • Journal of Magnetism and Magnetic Materials 310(2007), e821-e823
  • Vortrag (Konferenzbeitrag)
    International Conference on Magnetism (ICM 2006), IUPAP, 20.-25.08.2006, Kyoto, Japan

2006

Secondary structure and Pd(II) coordination in S-layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy

Fahmy, K.; Merroun, M.; Pollmann, K.; Raff, J.; Savchuk, O.; Hennig, C.; Selenska-Pobell, S.

Toxic avengers

Raff, J.; Selenska-Pobell, S.
  • Nuclear Engineering International 51(2006)619, 34-36

Manufacturing and characterization of Pd-nanoparticles formed on immobilized bacterial cells

Pollmann, K.; Merroun, M.; Raff, J.; Hennig, C.; Selenska-Pobell, S.
  • Letters in Applied Microbiology 43(2006)1, 39-45

Metal binding by bacteria from uranium mining waste piles and its technological applications

Pollmann, K.; Raff, J.; Merroun, M.; Fahmy, K.; Selenska-Pobell, S.

2005

Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements

Pollmann, K.; Raff, J.; Schnorpfeil, M.; Radeva, G.; Selenska-Pobell, S.

Complexation of Uranium by Cells and S-layer Sheets of Bacillus sphaericus JG-A12

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.

2004

Calcium dipicolinate induced germination of Bacillus spores embedded in thin silica layers: novel perspectives for the usage of biocers

Matys, S.; Raff, J.; Soltmann, U.; Selenska-Pobell, S.; Böttcher, H.; Pompe, W.

Bacteria from extreme environments: basis for new technical applications

Raff, J.; Selenska-Pobell, S.
  • BioMeT-Magazin (2004), 18

Interaction of U(VI) with bacterial strains isolated from uranium mining piles: spectroscopic and microscopic studies

Merroun, M.; Raff, J.; Rossberg, A.; Hennig, C.; Reich, T.; Selenska-Pobell, S.
  • Vortrag (Konferenzbeitrag)
    14th Goldschmidt Conference 2004, 05.-11.06.2004, Kopenhagen, Denmark
  • Geochimica et Cosmochimica Acta 68(2004)11, 499-499
  • Beitrag zu Proceedings
    14th Goldschmidt Conference, 05.-11.06.2004, Kopenhagen, Denmark
    Proceedings

Use of Bacteria for Cleaning Radioactive Wastewaters

Raff, J.; Selenska-Pobell, S.
  • Genomics/Proteomics Technology 22(2004)2, 32L-32N

2003

Biosorption of Heavy Metals by Sol-Gel Immobilized Bacillus sphaericus Cells, Spores and S-Layers

Soltmann, U.; Raff, J.; Selenska-Pobell, S.; Matys, S.; Pompe, W.; Böttcher, H.
  • Journal of Sol-Gel Science and Technology, 26: 1209-1212 (2003)

Biosorption of Uranium and Copper by Biocers

Raff, J.; Soltmann, U.; Matys, S.; Selenska-Pobell, S.; Pompe, W.; Böttcher, H.
  • Chemistry of Materials, 15 (1), 240-244, Jan. 2003

2001

A fast screening method for surface layers on Gram-positive bacteria

Wahl, R.; Raff, J.; Selenska-Pobell, S.; Mertig, M.; Pompe, W.
  • Biotechnology letters 23, 1485-1490 (2001)

Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S-layer of Bacillus sphaericus NCTC 9602

Wahl, R.; Mertig, M.; Raff, J.; Selenska-Pobell, S.; Pompe, W.
  • Advanced Materials 2001, 13, No. 10, May 17, p. 736-740

EXAFS investigation of uranium(VI) complexes formed at Bacillus cereus and Bacillus sphaericus surfaces

Hennig, C.; Selenska-Pobell, S.; Matz, W.; Panak, P.; Reich, T.; Roßberg, A.; Raff, J.; Bucher, J.; Bernhard, G.; Nitsche, H.
  • Radiochimica Acta 89 (2001), 625-631

2000

Complex formation of U(VI) with Bacillus-Isolates from a Uranium Mining Waste Pile

Panak, P.; Raff, J.; Selenska-Pobell, S.; Geipel, G.; Bernhard, G.; Nitsche, H.
  • Radiochimica Acta 88, 71-76 (2000)


Kontakt

Dr. Johannes Raff

Lei­ter Biogeochemie
j.raffAthzdr.de
Tel.: +49 351 260 2951