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Biogeochemie
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Kontakt

Dr. Johannes Raff

Lei­ter Biogeochemie
j.raffAthzdr.de
Tel.: +49 351 260 2951

Soziale Medien

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Wichtige Veröffentlichungen

2024

Biostimulation of Indigenous Microbes for Uranium Bioremediation in Former U Mine Water: Multidisciplinary approach assessment

Newman Portela, A.; Krawczyk-Bärsch, E.; Lopez Fernandez, M.; Bok, F.; Kassahun, A.; Drobot, B.; Steudtner, R.; Stumpf, T.; Raff, J.; Merroun, M. L.


2023

Learning from nature: recovery of rare earth elements by the extremophilic bacterium Methylacidiphilum fumariolicum

Singer, H.; Steudtner, R.; Sottorff, I.; Drobot, B.; Pol, A.; Op Den Camp, H. J. M.; Daumann, L. J.

Verknüpfte Publikationen


Uranium(VI) interactions with Pseudomonas sp. PS-0-L, V4-5-SB and T5-6-I

Kasko, J.; Li, X.; Müller, K.; Ge, Y.; Vettese, G. F.; Law, G. T. W.; Siitari-Kauppi, M.; Huittinen, N. M.; Raff, J.; Bomberg, M.; Herzig, M.


Equilibrium Thermodynamics of Macropa Complexes with Selected Metal Isotopes of Radiopharmaceutical Interest

Blei, M. K.; Waurick, L.; Reissig, F.; Kopka, K.; Stumpf, T.; Drobot, B.; Kretzschmar, J.; Mamat, C.


Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals

Hilpmann, S.; Moll, H.; Drobot, B.; Vogel, M.; Hübner, R.; Stumpf, T.; Cherkouk, A.

Verknüpfte Publikationen


Europium(III) Meets Etidronic Acid (HEDP): a Coordination Study Combining Spectroscopic, Spectrometric, and Quantum Chemical Methods

Heller, A.; Senwitz, C.; Foerstendorf, H.; Tsushima, S.; Holtmann, L.; Drobot, B.; Kretzschmar, J.


How tobacco (Nicotiana tabacum) BY-2 cells cope with Eu(III) – A microspectroscopic study.

Klotzsche, M.; Vogel, M.; Sachs, S.; Raff, J.; Stumpf, T.; Drobot, B.; Steudtner, R.


Halomonas gemina sp. nov. and Halomonas llamarensis sp. nov., two siderophore-producing organisms isolated from high-altitude salars of the Atacama Desert

Hintersatz, C.; Singh, S.; Antonio Rojas, L.; Kretzschmar, J.; Wei, T.-S.; Khambhati, K.; Kutschke, S.; Lehmann, F.; Singh, V.; Jain, R.; Pollmann, K.

Verknüpfte Publikationen


Distinct Effects of Chemical Toxicity and Radioactivity on Metabolic Heat of Cultured Cells Revealed by “Isotope-Editing”

Oertel, J.; Sachs, S.; Flemming, K.; Hassan Obeid, M.; Fahmy, K.


Eu(III) and Cm(III) Complexation by the Aminocarboxylates NTA, EDTA, and EGTA Studied with NMR, TRLFS, and ITC – An Improved Approach to More Robust Thermodynamics

Friedrich, S.; Sieber, C.; Drobot, B.; Tsushima, S.; Barkleit, A.; Schmeide, K.; Stumpf, T.; Kretzschmar, J.


Presence of uranium(V) during uranium(VI) reduction by Desulfosporosinus hippei DSM 8344T

Hilpmann, S.; Roßberg, A.; Steudtner, R.; Drobot, B.; Hübner, R.; Bok, F.; Prieur, D.; Bauters, S.; Kvashnina, K.; Stumpf, T.; Cherkouk, A.

Verknüpfte Publikationen


Minor Actinides Can Replace Essential Lanthanides in Bacterial Life

Singer, H.; Steudtner, R.; Klein, A. S.; Rulofs, C.; Zeymer, C.; Drobot, B.; Pol, A.; Martinez-Gomez, C.; Daumann, L. J.

Verknüpfte Publikationen


Localization and chemical speciation of europium(III) in Brassica napus plants

Jessat, J.; John, W.; Moll, H.; Vogel, M.; Steudtner, R.; Drobot, B.; Hübner, R.; Stumpf, T.; Sachs, S.


Environmental exposure to uranium in a population living in close proximity to gold mine tailings in South Africa

Zupunski, L.; Street, R.; Ostroumova, E.; Winde, F.; Sachs, S.; Geipel, G.; Nkosi, V.; Bouaoun, L.; Haman, T.; Schüz, J.; Mathee, A.


2022

Peptide functionalized Dynabeads for the magnetic carrier separation of rare-earth fluorescent lamp phosphors

Boelens, P.; Bobeth, C.; Hinman, N.; Weiß, S.; Zhou, S.; Vogel, M.; Drobot, B.; Shams Aldin Azzam, S.; Pollmann, K.; Lederer, F.


The Chemical Journey of Europium(III) through Winter Rye (Secale cereale L.) – Understanding through Mass Spectrometry and Chemical Microscopy

Stadler, J.; Vogel, M.; Steudtner, R.; Drobot, B.; Kogiomtzidis, A.; Weiss, M.; Walther, C.

Verknüpfte Publikationen


Vibrio salinus sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll in the western Pacific Ocean

Huang, W.; Wang, L.; Chen, J.; Chen, Y. C.; Wei, T.-S.; Chiang, Y. C.; Wang, P.; Lee, T.; Lin, S.; Huang, L.; Shieh, W. Y. S.


Lanmodulin peptides – unravelling the binding of the EF-Hand loop sequences stripped from the structural corset

Gutenthaler, S. M.; Tsushima, S.; Steudtner, R.; Gailer, M.; Hoffmann-Röder, A.; Drobot, B.; Daumann, L. J.

Verknüpfte Publikationen


Speciation and spatial distribution of Eu(III) in fungal mycelium

Günther, A.; Wollenberg, A.; Vogel, M.; Drobot, B.; Steudtner, R.; Freitag, L.; Hübner, R.; Stumpf, T.; Raff, J.


Peptidoglycan as major binding motif for Uranium bioassociation on Magnetospirillum magneticum AMB-1 in contaminated waters

Krawczyk-Bärsch, E.; Ramtke, J.; Drobot, B.; Müller, K.; Steudtner, R.; Kluge, S.; Hübner, R.; Raff, J.


A comprehensive study on the interaction of Eu(III) and U(VI) with plant cells (Daucus carota) in suspension

Jessat, J.; Moll, H.; John, W.; Bilke, M.-L.; Hübner, R.; Kretzschmar, J.; Steudtner, R.; Drobot, B.; Stumpf, T.; Sachs, S.


Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning

Iglesias-Artola, J. M.; Drobot, B.; Kar, M.; Fritsch, A. W.; Mutschler, H.; Dora Tang, T.-Y.; Kreysing, M.


Studies of Pyrroloquinoline Quinone Species in Solution and in Lanthanide-dependent Methanol Dehydrogenases

Al Danaf, N.; Kretzschmar, J.; Jahn, B.; Singer, H.; Pol, A.; Op Den Camp, H. J. M.; Steudtner, R.; Lamb, D. C.; Drobot, B.; Daumann, L. J.


Not just a background: pH buffers do interact with lanthanide ions – a Europium(III) case study

Mandal, P.; Kretzschmar, J.; Drobot, B.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Microscopic and spectroscopic bioassociation study of uranium(VI) with an archaeal Halobacterium isolate

Hilpmann, S.; Bader, M.; Steudtner, R.; Müller, K.; Stumpf, T.; Cherkouk, A.

Verknüpfte Publikationen


Endocytosis is a significant contributor to uranium(VI) uptake in tobacco (Nicotiana tabacum) BY-2 cells in phosphate-deficient culture

John, W.; Lückel, B.; Matschiavelli, N.; Hübner, R.; Matschi, S.; Hoehenwarter, W.; Sachs, S.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


New insights into U(VI) sorption onto montmorillonite from batch sorption and spectroscopic studies at increased ionic strength

Stockmann, M.; Fritsch, K.; Bok, F.; Marques Fernandes, M.; Baeyens, B.; Steudtner, R.; Müller, K.; Nebelung, C.; Brendler, V.; Stumpf, T.; Schmeide, K.


2021

Americium preferred: LanM, a natural lanthanide-binding protein favors an actinide over lanthanides

Singer, H.; Drobot, B.; Zeymer, C.; Steudtner, R.; Daumann, L.


Effect of temperature and cell viability on uranium biomineralization by the uranium mine isolate Penicillium simplicissimum

Schaefer, S.; Steudtner, R.; Hübner, R.; Krawczyk-Bärsch, E.; Merroun, M. L.


High-Gradient Magnetic Separation of Compact Fluorescent Lamp Phosphors: Elucidation of the Removal Dynamics in a Rotary Permanent Magnet Separator

Boelens, P.; Lei, Z.; Drobot, B.; Rudolph, M.; Li, Z.; Franzreb, M.; Eckert, K.; Lederer, F.


An updated strategic research agenda for the integration of radioecology in the european radiation protection research

Gilbin, R.; Arnold, T.; Beresford, N. A.; Berthomieu, C.; Brown, J. E.; de With, G.; Horemans, N.; Madruga, M. J.; Masson, O.; Merroun, M.; Michalik, B.; Muikku, M.; O’Toole, S.; Mrdakovic Popic, J.; Nogueira, P.; Real, A.; Sachs, S.; Salbu, B.; Stark, K.; Steiner, M.; Sweek, L.; Vandenhove, H.; Vidal, M.; Vives I. Batlle, J.


Curium(III) speciation in the presence of microbial cell wall components

Moll, H.; Barkleit, A.; Frost, L.; Raff, J.


Bentonite alteration in batch reactor experiments with and without fertilizing agents: implications for the disposal of radioactive waste

Podlech, C.; Matschiavelli, N.; Peltz, M.; Kluge, S.; Arnold, T.; Cherkouk, A.; Meleshyn, A.; Grathoff, G.; Warr, L. N.


Detecting bacterial cell viability in few μl solutions from impedance measurements on silicon‐based biochips

Bhat, V. J.; Vegesna, S. V.; Kiani, M.; Zhao, X.; Blaschke, D.; Du, N.; Vogel, M.; Kluge, S.; Raff, J.; Hubner, U.; Skorupa, I.; Rebohle, L.; Schmidt, H.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Quenching Mechanism of Uranyl(VI) by Chloride and Bromide in Aqueous and Non-Aqueous Solutions

Haubitz, T.; Drobot, B.; Tsushima, S.; Steudtner, R.; Stumpf, T.; Kumke, M. U.


Spatially resolved Eu(III) environments by chemical microscopy

Vogel, M.; Steudtner, R.; Fankhänel, T.; Raff, J.; Drobot, B.

Verknüpfte Publikationen


Investigation of the structure and dynamics of Gallium binding to high-affinity peptides elucidated by multi-scale simulation, quantum chemistry, NMR and ITC

Taylor, C. J.; Schönberger, N.; Laníková, A.; Patzschke, M.; Drobot, B.; Žídek, L.; Lederer, F.

Verknüpfte Publikationen


Microbial interaction with and tolerance of radionuclides: underlying mechanisms and biotechnological applications

Lopez Fernandez, M.; Jroundi, F.; Ruiz. Fresneda, M. A.; Merroun, M. L.


Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study

Pinel-Cabello, M.; Jroundi, F.; Lopez Fernandez, M.; Geffers, R.; Jarek, M.; Jauregui, R.; Link, A.; Vílchez-Vargas, R.; Merroun, M. L.


Curium(III) and europium(III) as luminescence probes for plant cell (Brassica napus) interactions with potentially toxic metals

Moll, H.; Schmidt, M.; Sachs, S.


Gallium-binding peptides as a tool for the sustainable treatment of industrial waste streams

Schönberger, N.; Taylor, C. J.; Schrader, M.; Drobot, B.; Matys, S.; Lederer, F.; Pollmann, K.


Uranium(VI) bioassociation by different fungi – a comparative study into molecular processes

Wollenberg, A.; Drobot, B.; Hübner, R.; Kretzschmar, J.; Freitag, L.; Lehmann, F.; Günther, A.; Stumpf, T.; Raff, J.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Sorption of Europium on Diatom Biosilica as Model of a “Green” Sorbent for f-Elements

Kammerlander, K. K. K.; Köhler, L.; Huittinen, N. M.; Bok, F.; Steudtner, R.; Oschatz, C.; Vogel, M.; Stumpf, T.; Brunner, E.


Uranium(VI) toxicity in tobacco BY-2 cell suspension culture - a physiological study

Rajabi, F.; Jessat, J.; Garimella, J. N.; Bok, F.; Steudtner, R.; Stumpf, T.; Sachs, S.


Bioassociation of U(VI) and Eu(III) by plant (Brassica napus) suspension cell cultures – A spectroscopic investigation

Jessat, J.; Sachs, S.; Moll, H.; John, W.; Steudtner, R.; Hübner, R.; Bok, F.; Stumpf, T.


Uranium and neptunium retention mechanisms in Gallionella ferruginea / ferrihydrite systems for remediation purposes

Krawczyk-Bärsch, E.; Scheinost, A. C.; Roßberg, A.; Müller, K.; Lehrich, J.; Bok, F.; Hallbeck, L.; Schmeide, K.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Dimeric and Trimeric Uranyl(VI)–Citrate Complexes in Aqueous Solution

Kretzschmar, J.; Tsushima, S.; Lucks, C.; Jäckel, E.; Meyer, R.; Steudtner, R.; Müller, K.; Roßberg, A.; Schmeide, K.; Brendler, V.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


2020

Presence of Bradyrhizobium sp. under continental conditions in Central Europe

Griebsch, A.; Matschiavelli, N.; Lewandowska, S.; Schmidtke, K.


An integrated approach combining soil profile, records and tree ring analysis to identify the origin of environmental contamination in a former uranium mine (Rophin, France)

Martin, A.; Hassan-Loni, Y.; Fichtner, A.; Péron, O.; David, K.; Chardon, P.; Larrue, S.; Gourgiotis, A.; Sachs, S.; Arnold, T.; Grambow, B.; Stumpf, T.; Montavon, G.


Trimeric uranyl(VI)–citrate forms Na+, Ca2+, and La3+ sandwich complexes in aqueous solution

Kretzschmar, J.; Tsushima, S.; Drobot, B.; Steudtner, R.; Schmeide, K.; Stumpf, T.

Verknüpfte Publikationen


Temperature-dependent luminescence spectroscopic investigations of U(VI) complexation with the halides F- and Cl-

Demnitz, M.; Hilpmann, S.; Lösch, H.; Bok, F.; Steudtner, R.; Patzschke, M.; Stumpf, T.; Huittinen, N. M.


Disturbing-free determination of yeast concentration in DI water and in glucose using impedance biochips

Kiani, M.; Du, N.; Vogel, M.; Raff, J.; Hübner, U.; Skorupa, I.; Bürger, D.; Schulz, S. E.; Schmidt, O. G.; Blaschke, D.; Schmidt, H.


Molecular binding of Eu(III)/Cm(III) by Stenotrophomonas bentonitica and its impact on the safety of future geodisposal of radioactive waste

Ruiz-Fresneda, M. A.; Lopez Fernandez, M.; Martinez-Moreno, M. F.; Cherkouk, A.; Ju-Nam, Y.; Ojeda, J. J.; Moll, H.; Merroun, M. L.


Interaction of curium(III) with surface-layer proteins from Lysinibacillus sphaericus JG-A12

Moll, H.; Lehmann, F.; Raff, J.


Signatures of Technetium Oxidation States: A New Approach

Bauters, S.; Scheinost, A.; Schmeide, K.; Weiß, S.; Dardenne, K.; Rothe, J.; Mayordomo, N.; Steudtner, R.; Stumpf, T.; Abram, U.; Butorin, S.; Kvashnina, K.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Temperature‒dependent luminescence spectroscopic and mass spectrometric investigations of U(VI) complexation with aqueous silicates in the acidic pH‒range

Lösch, H.; Raiwa, M.; Jordan, N.; Steppert, M.; Steudtner, R.; Stumpf, T.; Huittinen, N. M.


Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure

Moll, H.; Sachs, S.; Geipel, G.


Uranium(VI) Complexes of Glutathione Disulfide Forming in Aqueous Solution

Kretzschmar, J.; Strobel, A.; Haubitz, T.; Drobot, B.; Steudtner, R.; Barkleit, A.; Brendler, V.; Stumpf, T.

Verknüpfte Publikationen


2019

Human exposure to uranium in South African gold mining areas using barber-based hair sampling

Winde, F.; Geipel, G.; Espina, C.; Schüz, J.


How do actinyls interact with hyperphosphorylated yolk protein Phosvitin ?

Kumar, S.; Creff, G.; Hennig, C.; Rossberg, A.; Steudtner, R.; Raff, J.; Vidaud, C.; Oberhaensli, F. R.; Bottein, Y.; Den Auwer, C.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


The year-long development of microorganisms in uncompacted Bavarian bentonite slurries at 30 °C and 60 °C

Matschiavelli, N.; Kluge, S.; Podlech, C.; Standhaft, D.; Grathoff, G.; Ikeda-Ohno, A.; Warr, L.; Chukharkina, A.; Arnold, T.; Cherkouk, A.


Chromatopanning for the identification of gallium binding peptides

Schönberger, N.; Braun, R.; Matys, S.; Lederer, F. L.; Lehmann, F.; Flemming, K.; Pollmann, K.


Thermodynamic and structural studies on the Ln(III)/An(III) malate complexation

Taube, F.; Drobot, B.; Roßberg, A.; Foerstendorf, H.; Acker, M.; Patzschke, M.; Trumm, M.; Taut, S.; Stumpf, T.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon

Bader, M.; Moll, H.; Steudtner, R.; Lösch, H.; Drobot, B.; Stumpf, T.; Cherkouk, A.


Microbial diversity in an arid, naturally saline environment

Bachran, M.; Kluge, S.; Lopez-Fernandez, M.; Cherkouk, A.


Synthesis, 18F-labelling and radiopharmacological characterisation of the C-terminal 30mer of Clostridium perfringens enterotoxin as a potential claudin-targeting peptide

Löser, R.; Bader, M.; Kuchar, M.; Wodtke, R.; Lenk, J.; Wodtke, J.; Kuhne, K.; Bergmann, R.; Haase-Kohn, C.; Urbanová, M.; Steinbach, J.; Pietzsch, J.


Reversible pH-dependent curium(III) biosorption by the bentonite yeast isolate Rhodotorula mucilaginosa BII-R8

Lopez-Fernandez, M.; Moll, H.; Merroun, M. L.


Cm3+/ Eu3+ Induced Structural, Mechanistic and Functional Implications for Calmodulin

Drobot, B.; Schmidt, M.; Mochizuki, Y.; Abe, T.; Okuwaki, K.; Brulfert, F.; Falke, S.; Samsonov, S.; Komeiji, Y.; Betzel, C.; Stumpf, T.; Raff, J.; Tsushima, S.


S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Jung, J.; Lakatos, M.; Bengs, S.; Matys, S.; Raff, J.; Blüher, A.; Cuniberti, G.


2018

Depth and Dissolved Organic Carbon Shape Microbial Communities in Surface Influenced but Not Ancient Saline Terrestrial Aquifers

Lopez-Fernandez, M.; Åström, M.; Bertilsson, S.; Dopson, M.


Microbial Community and Metabolic Activity in Thiocyanate Degrading Low Temperature Microbial Fuel Cells

Ni, G.; Canizales, S.; Broman, E.; Simone, D.; Palwai, V. R.; Lundin, D.; Lopez-Fernandez, M.; Sleutels, T.; Dopson, M.


Metatranscriptomes Reveal That All Three Domains of Life Are Active but Are Dominated by Bacteria in the Fennoscandian Crystalline Granitic Continental Deep Biosphere

Lopez-Fernandez, M.; Simone, D.; Wu, X.; Soler, L.; Nilsson, E.; Holmfeldt, K.; Lantz, H.; Bertilsson, S.; Dopson, M.


Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling

Lopez-Fernandez, M.; Broman, E.; Turner, S.; Wu, X.; Bertilsson, S.; Dopson, M.


Impact of Haloarchaea on speciation of uranium – a multi-spectroscopic approach

Bader, M.; Rossberg, A.; Steudtner, R.; Drobot, B.; Großmann, K.; Schmidt, M.; Musat, N.; Stumpf, T.; Ikeda-Ohno, A.; Cherkouk, A.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Ultrafast transient absorption spectroscopy of UO22+ and [UO2Cl]+

Haubitz, T.; Tsushima, S.; Steudtner, R.; Drobot, B.; Geipel, G.; Stumpf, T.; Kumke, M. U.


Effect of U(VI) aqueous speciation on the binding of uranium by the cell surface of Rhodotorula mucilaginosa, a natural yeast isolate from bentonites

Lopez-Fernandez, M.; Romero-Gonzalez, M.; Günther, A.; Solari, P. L.; Merroun, M. L.


Metabolism-dependent bioaccumulation of uranium by Rhodosporidium toruloides isolated from the flooding water of a former uranium mine

Gerber, U.; Hübner, R.; Rossberg, A.; Krawczyk-Bärsch, E.; Merroun, M. L.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Removal and recovery of uranium by waste digested activated sludge in fed-batch stirred tank reactor

Jain, R.; Peräniemi, S.; Jordan, N.; Vogel, M.; Weiss, S.; Foerstendorf, H.; Lakaniemi, A. M.


Bio-recycling of metals: Recycling of technical products using biological applications

Pollmann, K.; Kutschke, S.; Matys, S.; Raff, J.; Hlawacek, G.; Lederer, F. L.


Multidisciplinary characterization of U(VI) sequestration by Acidovorax facilis for bioremediation purposes

Krawczyk-Bärsch, E.; Gerber, U.; Müller, K.; Moll, H.; Rossberg, A.; Steudtner, R.; Merroun, M.

Beteiligte Forschungsanlagen

Verknüpfte Publikationen


Comparative analysis of uranium bioassociation with halophilic bacteria and archaea

Bader, M.; Müller, K.; Foerstendorf, H.; Schmidt, M.; Simmons, K.; Swanson, J. S.; Reed, D. T.; Stumpf, T.; Cherkouk, A.


Biotransformation and detoxification of selenite by microbial biogenesis of selenium-sulfur nanoparticles

Vogel, M.; Fischer, S.; Maffert, A.; Hübner, R.; Scheinost, A.; Franzen, C.; Steudtner, R.

Beteiligte Forschungsanlagen

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